Jin Fangfang, Zeng Qian, Qian Husun, Zhang Dian, Wei Yu, Wang Yange, Chai Chengsen, Cheng Wei, Ding Shijia, Chen Tingmei
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, China.
The Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Front Pharmacol. 2022 Feb 23;13:807498. doi: 10.3389/fphar.2022.807498. eCollection 2022.
Chemotherapy, as one of the principal modalities for cancer therapy, is limited by its non-specific and inefficient delivery to tumors. To overcome these limitations, we report herein a dual-targeted aptamer-decorated DNA hydrogel system (DTA-H) to achieve efficient, stable, and targeted delivery of drugs. Firstly, DNA hydrogel was formed by the rolling circle amplification. By reasonable design, double target and multivalent aptamers were decorated on DNA hydrogel to load DOX. The results confirmed that DTA-H can deliver chemotherapy drugs and aptamer nucleic acids drugs to target cells, inducing degradation of HER2 protein while chemotherapy is synergistic to inhibit HER2-positive breast cancer growth. The proposed drug delivery system has significant potential to achieve efficient, stable, and targeted delivery of drugs and cancer therapy.
化疗作为癌症治疗的主要方式之一，受到其对肿瘤非特异性和低效递送的限制。为了克服这些限制，我们在此报告一种双靶向适体修饰的DNA水凝胶系统（DTA-H），以实现药物的高效、稳定和靶向递送。首先，通过滚环扩增形成DNA水凝胶。通过合理设计，在DNA水凝胶上修饰双靶点和多价适体以负载阿霉素。结果证实，DTA-H可以将化疗药物和适体核酸药物递送至靶细胞，诱导HER2蛋白降解，同时化疗协同抑制HER2阳性乳腺癌生长。所提出的药物递送系统在实现药物的高效、稳定和靶向递送以及癌症治疗方面具有巨大潜力。
