Department of Hematology, Shanxi Children's Hospital, China.
Scientific Research Department of Clinical Laboratory Center of Shanxi Children's Hospital, China.
Comput Math Methods Med. 2022 Mar 4;2022:8955322. doi: 10.1155/2022/8955322. eCollection 2022.
To investigate the role and mechanism of lncRNA-pvt1 in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
The expression of lncRNA-pvt1 in bone marrow tissues of ALL patients after initial diagnosis and complete remission was detected by RT-PCR to explore its possible involvement in the pathogenesis of ALL. The proliferation and apoptosis of Jurkat cells transfected with lncRNA-pvt1 were observed by MTT and flow cytometry.
lncRNA-pvt1 expression was upregulated in bone marrow of ALL patients. Knockdown of lncRNA-pvt1 inhibited Jurkat cell proliferation and increased its apoptosis rate.
Silencing lncRNA-pvt1 expression can inhibit the development of ALL.
研究长链非编码 RNA-pvt1 在儿童急性淋巴细胞白血病（ALL）发病机制中的作用及机制。
采用 RT-PCR 检测初诊和完全缓解后 ALL 患者骨髓组织中 lncRNA-pvt1 的表达，探讨其在 ALL 发病机制中的可能作用。通过 MTT 和流式细胞术观察转染 lncRNA-pvt1 后 Jurkat 细胞的增殖和凋亡情况。
lncRNA-pvt1 在 ALL 患者骨髓中表达上调。沉默 lncRNA-pvt1 表达可抑制 Jurkat 细胞增殖，增加其凋亡率。
沉默 lncRNA-pvt1 表达可抑制 ALL 的发生发展。
