School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang, China.
Sci Rep. 2019 Jan 24;9(1):548. doi: 10.1038/s41598-018-36985-x.
To analyze the level and diagnostic value of plasmacytoma variant translocation 1 (PVT1) in gastric cancer (GC) of Han and Uygur in Xinjiang, China, we collected 42 GC and 47 normal gastric tissues and performed tissue microarray. In situ hybridization was used to detect PVT1, while immunohistochemistry was used to analyze c-myc. The relationship between PVT1, c-myc and clinical pathological features was investigated. We then analyzed the expression of PVT1 in six GC cell lines. RNA interference was used to silence PVT1 in BGC823 and AGS cells. c-myc was detected by western blotting after silencing PVT1, while proliferation, invasion and migration ability were also analyzed. We found that PVT1 and c-myc were highly expressed in both Han and Uygur GC tissues. In Han GC, PVT1 was correlated with lymph node metastasis and primary tumor site. In Uygur GC, both PVT1 and c-myc were correlated with lymph node metastasis and clinical staging. PVT1 was positively correlated with c-myc. BGC823 and AGS cells exhibited high levels of PVT1. When PVT1 expression was silenced, the expression of c-myc decreased, while migration and invasion ability were also decreased in cells. PVT1 could therefore be a potential biomarker to predict the metastatic tendency of GC in both Han and Uygur patients.
为分析中国人维吾尔族浆细胞瘤变异易位 1（PVT1）在新疆汉族和维吾尔族胃癌（GC）中的水平和诊断价值，我们收集了 42 例 GC 组织和 47 例正常胃组织，并进行了组织微阵列分析。采用原位杂交法检测 PVT1，免疫组化法分析 c-myc。分析 PVT1、c-myc 与临床病理特征的关系。然后分析了 PVT1 在六种 GC 细胞系中的表达。用 RNA 干扰沉默 BGC823 和 AGS 细胞中的 PVT1。沉默 PVT1 后，通过 Western blot 检测 c-myc 的表达，同时分析增殖、侵袭和迁移能力。结果发现，PVT1 和 c-myc 在汉族和维吾尔族 GC 组织中均高表达。在汉族 GC 中，PVT1 与淋巴结转移和原发肿瘤部位有关。在维吾尔族 GC 中，PVT1 和 c-myc 均与淋巴结转移和临床分期有关。PVT1 与 c-myc 呈正相关。BGC823 和 AGS 细胞中 PVT1 表达水平较高。沉默 PVT1 表达后，细胞中 c-myc 表达降低，迁移和侵袭能力也降低。因此，PVT1 可能是汉族和维吾尔族 GC 患者预测转移倾向的潜在生物标志物。
