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长链非编码 RNA GJA9-MYCBP 和 PVT1 的上调是急性淋巴细胞白血病的潜在诊断生物标志物。

Upregulation of the long noncoding RNA GJA9-MYCBP and PVT1 is a potential diagnostic biomarker for acute lymphoblastic leukemia.

机构信息

Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Thalassemia & Hemoglobinopathy Research center, Health research institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

出版信息

Cancer Rep (Hoboken). 2024 Jul;7(7):e2115. doi: 10.1002/cnr2.2115.

DOI:10.1002/cnr2.2115

PMID:38994720

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11240143/

Abstract

BACKGROUND

Acute lymphoblastic leukemia (ALL) is the most common type of blood cancer in children. Aberrant expression of long noncoding RNAs (lncRNAs) may set stages for ALL development. LncRNAs are emerging as a novel diagnostic and prognostic biomarker for ALL. Herein, we aimed to evaluate the expression of lncRNA GJA9-MYCBP and PVT1 in blood samples of ALL and healthy individuals.

METHODS

As a case-control study, 40 pairs of ALL and healthy individual samples were used. The expression of MYC and each candidate lncRNA was measured using quantitative real-time PCR. Any possible association between the expression of putative noncoding RNAs and clinicopathological characteristics was also evaluated.

RESULTS

LncRNA GJA9-MYCBP and PVT1 were significantly upregulated in ALL samples compared with healthy ones. Similarly, mRNA levels of MYC were increased in ALL samples than control ones. Receiver operating characteristic curve analysis indicated a satisfactory diagnostic efficacy (p-value <.0001), suggesting that lncRNA GJA9-MYCBP and PVT1 may serve as a diagnostic biomarker for ALL. Linear regression analysis unveiled positive correlations between the expression level of MYC and lncRNA GJA9-MYCBP and PVT1 in ALL patients (p-values <.01).

CONCLUSIONS

In this study, we provided approval for the clinical diagnostic significance of lncRNA GJA9-MYCBP and PVT1that their upregulations may be a diagnostic biomarker for ALL.

摘要

背景

急性淋巴细胞白血病（ALL）是儿童中最常见的血液癌症类型。长非编码 RNA（lncRNA）的异常表达可能为 ALL 的发展奠定了基础。lncRNA 正在成为 ALL 的一种新的诊断和预后生物标志物。在此，我们旨在评估 lncRNA GJA9-MYCBP 和 PVT1 在 ALL 和健康个体血液样本中的表达。

方法

作为病例对照研究，使用了 40 对 ALL 和健康个体样本。使用实时定量 PCR 测量 MYC 和每个候选 lncRNA 的表达。还评估了潜在非编码 RNA 的表达与临床病理特征之间的任何可能关联。

结果

与健康个体相比，lncRNA GJA9-MYCBP 和 PVT1 在 ALL 样本中显著上调。同样，ALL 样本中的 MYC mRNA 水平高于对照样本。受试者工作特征曲线分析表明具有令人满意的诊断效能（p 值 <.0001），表明 lncRNA GJA9-MYCBP 和 PVT1 可能作为 ALL 的诊断生物标志物。线性回归分析揭示了 ALL 患者中 MYC 和 lncRNA GJA9-MYCBP 与 PVT1 表达水平之间的正相关关系（p 值 <.01）。