鼠伤寒沙门氏菌中霍乱相关肠毒素的竞争性酶联免疫吸附测定
Competitive enzyme-linked immunosorbent assay for cholera-related enterotoxins in Salmonella typhimurium.
作者信息
Hariharan H, Booth B A, Brickman T J, Katt W C, Boesman-Finkelstein M, Finkelstein R A
出版信息
J Clin Microbiol. 1986 Aug;24(2):298-300. doi: 10.1128/jcm.24.2.298-300.1986.
Abstract
We report a rapid competitive enzyme-linked immunosorbent assay to screen Salmonella typhimurium strains for cholera-related enterotoxin antigens. Polymyxin B extracts of bacterial cells from syncase-glucose broth cultures of 7 of 15 strains gave positive results. The specificity of the test was confirmed with known heat-labile-enterotoxin-positive and -negative Escherichia coli strains which gave significantly different values.
摘要
我们报告了一种快速竞争性酶联免疫吸附测定法，用于筛选鼠伤寒沙门氏菌菌株中的霍乱相关肠毒素抗原。15株菌株中的7株在合酶 - 葡萄糖肉汤培养物中的细菌细胞多粘菌素B提取物检测结果呈阳性。用已知的热不稳定肠毒素阳性和阴性大肠杆菌菌株进行验证，结果显示有显著差异，从而证实了该测试的特异性。
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