Cheng Gong, Zhang Ji, Jia Shuo, Feng Panpan, Chang Fengjun, Yan Li, Gupta Pranay, Wu Haoyu
Department of Cardiology, Shaanxi Provincial People's Hospital, Xian, 710068, People's Republic of China.
Department of Emergency, Shaanxi Provincial People's Hospital, Xian, 710068, People's Republic of China.
J Inflamm Res. 2022 Mar 5;15:1637-1651. doi: 10.2147/JIR.S348883. eCollection 2022.
Myocardial ischemic/reperfusion (I/R) injury is a key prognostic factor after the myocardial infarction. However, at the time of reperfusion, the myocardial tissue has undergone for the necrosis and initiated the induction of oxidative stress and inflammation. The current study was to scrutinize the cardioprotective effect of gossypin against ISO-induced I/R injury in myocardial tissue and explore the possible underlying mechanism.
Sprague Dawley (SD) was used in the current protocol and ISO was used for induction the I/R in rat. The rats were divided into different groups and received the oral administration of gossypin treatment before the reperfusion. The body weight, heart weight and heart body weight ratio were estimated. The antioxidant, cardiac injury parameters, inflammatory cytokines, inflammatory mediators, gut microbiota and lipid parameters were estimated. At the end, heart tissue histopathological study was carried out.
ISO-induced I/R rats received the gossypin treatment significantly (P < 0.001) enhanced the body weight and decreased the heart weight, along with suppressed the infarct size. Gossypin treatment significantly (P < 0.001) reduced the level of heart parameters, such as creatinine kinase-MB (CK-MB), lactate dehydrogenase (LDH), creatine kinase (CK), cardiac troponin I (CTn-I) and cardiac troponin T (CTn-T) in the serum. Gossypin treatment significantly (P < 0.001) altered the cardiac function, hepatic, antioxidant, inflammatory cytokines and inflammatory mediators. Gossypin significantly (P < 0.001) suppressed the MMP-2 and MMP-9 in ISO-induced I/R rats. Gossypin treatment considerably alleviated the gut dysbiosis through altered to (F/B) ratio and also maintained the relative abundance of and XIVs.
Based on result, we can conclude that gossypin is an alternative drug for the treatment of ISO-induced I/R in rats via alteration of oxidative stress, inflammatory reaction and gut microbiota.
心肌缺血/再灌注（I/R）损伤是心肌梗死后的关键预后因素。然而，在再灌注时，心肌组织已经发生坏死并引发氧化应激和炎症反应。本研究旨在探讨棉子苷对异丙肾上腺素（ISO）诱导的心肌组织I/R损伤的心脏保护作用，并探索其可能的潜在机制。
本实验采用Sprague Dawley（SD）大鼠，使用ISO诱导大鼠发生I/R。将大鼠分为不同组，并在再灌注前口服棉子苷进行治疗。评估大鼠体重、心脏重量和心脏体重比。检测抗氧化指标、心脏损伤参数、炎症细胞因子、炎症介质、肠道微生物群和脂质参数。最后，进行心脏组织病理研究。
接受棉子苷治疗的ISO诱导I/R大鼠体重显著增加（P < 0.001），心脏重量减轻，梗死面积减小。棉子苷治疗显著降低了血清中心脏参数水平，如肌酸激酶同工酶-MB（CK-MB）、乳酸脱氢酶（LDH）、肌酸激酶（CK）、心肌肌钙蛋白I（CTn-I）和心肌肌钙蛋白T（CTn-T）（P < 0.001）。棉子苷治疗显著改变了心脏功能、肝脏功能、抗氧化指标、炎症细胞因子和炎症介质（P < 0.001）。棉子苷显著抑制了ISO诱导I/R大鼠中的基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）（P < 0.001）。棉子苷治疗通过改变厚壁菌门与拟杆菌门（F/B）比值，显著缓解了肠道菌群失调，并维持了双歧杆菌属和阿克曼菌属XIVs的相对丰度。
基于结果，我们可以得出结论，棉子苷可通过改变氧化应激、炎症反应和肠道微生物群，作为治疗大鼠ISO诱导I/R的替代药物。
