Department of Oral Radiology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Molecular and Biomolecular Physics, National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania.
Int J Oral Maxillofac Surg. 2022 Nov;51(11):1373-1381. doi: 10.1016/j.ijom.2022.02.015. Epub 2022 Mar 10.
Due to the high incidence of head and neck cancer and under-diagnosis in the early stages, non-invasive and highly accurate diagnostic tests are required for cancer detection. Recent advances in Raman spectroscopy techniques have yielded promising sensitivity and specificity results in the evaluation of cancer. The aim of this study was to investigate the potential value of Raman spectroscopy in oral cavity and oropharyngeal cancer diagnosis based on currently available scientific papers. A search of the PubMed database was performed using a specific strategy and according to the PRISMA guidelines. Raman spectroscopy achieved a maximum accuracy of 98% in cancer detection, while accuracy was 97.24% for tumour grading evaluation, 95% for cancer treatment assessment, and 77% for the detection of cancer recurrence. Moreover, early-stage cancer can be identified by Raman spectroscopy investigation of liquid biopsy samples. An in vivo technique with direct mucosa examination by fibre-optic Raman spectroscopy obtained a maximum accuracy of 94% in cancer diagnosis. The most prominent markers of the presence of malignancy were an increase in Raman signal intensity for proteins, nucleic acids, and water and a decrease for lipids. These cancer discriminants were detected in both fingerprint and high wavenumber regions. In conclusion, Raman spectroscopy is a promising tool for oral cavity and oropharyngeal cancer screening.
由于头颈部癌症的发病率高且早期诊断不足，因此需要非侵入性且高度准确的诊断测试来进行癌症检测。拉曼光谱技术的最新进展在癌症评估中取得了有前途的灵敏度和特异性结果。本研究旨在根据现有科学文献探讨拉曼光谱在口腔和口咽癌诊断中的潜在价值。使用特定策略并根据 PRISMA 指南对 PubMed 数据库进行了检索。拉曼光谱在癌症检测中的最大准确率达到 98%，而在肿瘤分级评估中的准确率为 97.24%，在癌症治疗评估中的准确率为 95%，在癌症复发检测中的准确率为 77%。此外，通过对液体活检样本进行拉曼光谱研究可以识别早期癌症。光纤拉曼光谱对直接粘膜检查的体内技术在癌症诊断中获得了 94%的最高准确率。存在恶性肿瘤的最突出标志物是蛋白质、核酸和水的拉曼信号强度增加，而脂质则减少。这些癌症判别因子在指纹和高波数区域均被检测到。总之，拉曼光谱是口腔和口咽癌筛查的一种有前途的工具。
