A sandwich enzyme immunoassay for wheat gliadin. - Suppr | 超能文献

A sandwich enzyme immunoassay for wheat gliadin.

作者信息

Troncone R, Vitale M, Donatiello A, Farris E, Rossi G, Auricchio S

出版信息

J Immunol Methods. 1986 Aug 21;92(1):21-3. doi: 10.1016/0022-1759(86)90498-9.

DOI:10.1016/0022-1759(86)90498-9

Abstract

A sandwich double antibody solid-phase enzyme immunoassay (EIA) that can detect and quantitate gliadin is described. Quantitative analysis could be carried out in the range of 5-400 ng/ml and the assay was more sensitive than inhibition EIA. Gliadin possessed weak cross-reactivity with oats, maize and rice prolamines. In the presence of anti-gliadin antibody (serum from a patient with active coeliac disease) gliadin could not be detected, but the sensitivity of the assay was restored by heat treatment of the serum. This EIA can therefore be employed to assess the gliadin content of biological fluids where specific antibody may coexist.

摘要

相似文献

1

A sandwich enzyme immunoassay for wheat gliadin.

J Immunol Methods. 1986 Aug 21;92(1):21-3. doi: 10.1016/0022-1759(86)90498-9.

2

Monoclonal antibody ELISA to quantitate wheat gliadin contamination of gluten-free foods.

J Immunol Methods. 1987 Apr 2;98(1):123-7. doi: 10.1016/0022-1759(87)90445-5.

3

Enzyme-linked immunosorbent assay for quantitation of cereal proteins toxic in coeliac disease.

Clin Chim Acta. 1988 Dec 30;178(3):261-70. doi: 10.1016/0009-8981(88)90234-3.

4

Measurement of gluten using a monoclonal antibody to a sequenced peptide of alpha-gliadin from the coeliac-activating domain I.

J Biochem Biophys Methods. 1994 Jan;28(1):77-82. doi: 10.1016/0165-022x(94)90066-3.

5

Passage of gliadin into human breast milk.

Acta Paediatr Scand. 1987 May;76(3):453-6. doi: 10.1111/j.1651-2227.1987.tb10498.x.

6

Wheat gliadin fractions and other cereal antigens reactive with antibodies in the sera of coeliac patients.

Clin Exp Immunol. 1982 Dec;50(3):651-60.

7

A radioimmunoassay for wheat gliadin to assess the suitability of gluten free foods for patients with coeliac disease.

Clin Exp Immunol. 1985 Mar;59(3):703-8.

8

An analysis of cereals that react with serum antibodies in patients with coeliac disease.

J Pediatr Gastroenterol Nutr. 1987 May-Jun;6(3):346-50. doi: 10.1097/00005176-198705000-00007.

9

[Characteristics of gliadin and antibodies against gliadin in celiac disease].

Acta Paediatr Acad Sci Hung. 1977;18(2):155-66.

10

Innovative approach to low-level gluten determination in foods using a novel sandwich enzyme-linked immunosorbent assay protocol.

Eur J Gastroenterol Hepatol. 2003 May;15(5):465-74. doi: 10.1097/01.meg.0000059119.41030.df.

引用本文的文献

1

Review of methods for measuring gliadins in food.

Gut. 1990 Jun;31(6):712-3. doi: 10.1136/gut.31.6.712.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。