State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, P.R. China.
Key Laboratory for Agro-Biodiversity and Pest Control (Ministry of Education), College of Plant Protection, Yunnan Agricultural University, Kunming 650201, P.R. China.
J Microbiol Biotechnol. 2022 Mar 28;32(3):294-301. doi: 10.4014/jmb.2112.12005.
In our greenhouse experiment, soil heat treatment groups (50, 80, and 121°C) significantly promoted growth and disease suppression of in consecutively cultivated soil (CCS) samples ( < 0.01), and 80°C worked better than 50°C and 121°C ( < 0.01). Furthermore, we found that heat treatment at 80°C changes the microbial diversity in CCS, and the inhibition ratios of culturable microorganisms, such as fungi and actinomycetes, were nearly 100%. However, the heat-tolerant bacterial community was preserved. The 16S rRNA gene and internal transcribed spacer (ITS) sequencing analyses indicated that the soil heat treatment had a greater effect on the Chao1 index and Shannon's diversity index of bacteria than fungi, and the relative abundances of Firmicutes and Proteobacteria were significantly higher than without heating (80 and 121°C, < 0.05). Soil probiotic bacteria, such as (67%), (9%), (6%), (6%), and (4%), remained in the soil after the 80°C and 121°C heat treatments. Although steam increased the relative abundances of most of the heat-tolerant microbes before sowing, richness and diversity gradually recovered to the level of CCS, regardless of fungi or bacteria, after replanting. Thus, we added heat-tolerant microbes (such as ) after steaming, which reduced the relative abundance of pathogens, recruited antagonistic bacteria, and provided a long-term protective effect compared to the steaming and alone ( < 0.05). Taken together, the current study provides novel insight into sustainable agriculture in a consecutively cultivated system.
在我们的温室实验中，土壤热处理组（50、80 和 121°C）显著促进了连续种植土壤（CCS）样本中 的生长和病害抑制（<0.01），并且 80°C 比 50°C 和 121°C 效果更好（<0.01）。此外，我们发现 80°C 的热处理改变了 CCS 中的微生物多样性，可培养微生物（如真菌和放线菌）的抑制率接近 100%。然而，耐热细菌群落得以保留。16S rRNA 基因和内部转录间隔区（ITS）测序分析表明，土壤热处理对细菌的 Chao1 指数和 Shannon 多样性指数的影响大于真菌，厚壁菌门和变形菌门的相对丰度明显高于未加热处理（80 和 121°C，<0.05）。土壤益生菌，如 （67%）、 （9%）、 （6%）、 （6%）和 （4%），在 80°C 和 121°C 热处理后仍留在土壤中。虽然蒸汽在播种前增加了大多数耐热微生物的相对丰度，但无论真菌还是细菌，在重新种植后，丰富度和多样性逐渐恢复到 CCS 的水平。因此，我们在蒸汽处理后添加了耐热微生物（如 ），与单独蒸汽和 处理相比，减少了病原菌的相对丰度，招募了拮抗细菌，并提供了长期的保护作用（<0.05）。综上所述，本研究为连续种植系统中的可持续农业提供了新的见解。
