Evaluation of the Altered Tissue Expression of and Genes in Oral and Cutaneous Lichen Planus Compared to Normal Healthy Tissues.

BACKGROUND

Recent highlights have investigated the possible roles of molecular chaperons like heat shock proteins (HSPs) into Lichen Planus (LP)-onset and pathogenesis. This study for the first, determine the expression of both and genes in cutaneous LP (CLP) and oral LP (OLP) lesions compared to normal healthy cases and between different subtypes of OLP lesions by real-time (RT)-PCR.

MATERIALS AND METHODS

Paraffin blocks of LP lesions including 56 OLP and 56 CLP samples were selected from theMashhad University of Medical sciences, Mashhad, Iran. Also 56 biopsy samples of healthy normal participants were selected. The demographic and clinical characteristics were extracted from their medical records. The expression of and HPS70 genes were evaluated using the real-time RT-PCR method.

RESULTS

The comparison of the expression of and genes among the patients with CLP and OLP showed a significant overexpression of and genes in both groups compared to the normal participants ( = 0.001). The expression of and genes was high in both the groups of CLP and OLP patients, but the amount was not significantly different between the two groups. Comparing the two mucosal subgroups of OLP lesions (non-erosive and erosive) showed that the expression of the and in erosive subtypes of OLP was significantly higher than the non-erosive subtypes of OLP ( = 0.001).

CONCLUSION

Regarding the overexpression of and in the LP lesions compared to healthy biopsies, we conclude that and could have key roles in the etiopathogenesis of the OLP and CLP lesions. The overexpression of both and in the erosive OLP group compared to the non-erosive OLP group emphasized the possible roles of HSPs in the pathogenesis and premalignant changes of OLP lesions.