Mohtasham Nooshin, Shahabinejad Mehdi, Kafiroudi Somayeh, Mohajertehran Farnaz
Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Oral and Maxillofacial Pathology, School of Dentistry, Mashhad, Iran.
Indian J Dermatol. 2021 Nov-Dec;66(6):591-597. doi: 10.4103/ijd.ijd_1060_20.
Recent highlights have investigated the possible roles of molecular chaperons like heat shock proteins (HSPs) into Lichen Planus (LP)-onset and pathogenesis. This study for the first, determine the expression of both and genes in cutaneous LP (CLP) and oral LP (OLP) lesions compared to normal healthy cases and between different subtypes of OLP lesions by real-time (RT)-PCR.
Paraffin blocks of LP lesions including 56 OLP and 56 CLP samples were selected from theMashhad University of Medical sciences, Mashhad, Iran. Also 56 biopsy samples of healthy normal participants were selected. The demographic and clinical characteristics were extracted from their medical records. The expression of and HPS70 genes were evaluated using the real-time RT-PCR method.
The comparison of the expression of and genes among the patients with CLP and OLP showed a significant overexpression of and genes in both groups compared to the normal participants ( = 0.001). The expression of and genes was high in both the groups of CLP and OLP patients, but the amount was not significantly different between the two groups. Comparing the two mucosal subgroups of OLP lesions (non-erosive and erosive) showed that the expression of the and in erosive subtypes of OLP was significantly higher than the non-erosive subtypes of OLP ( = 0.001).
Regarding the overexpression of and in the LP lesions compared to healthy biopsies, we conclude that and could have key roles in the etiopathogenesis of the OLP and CLP lesions. The overexpression of both and in the erosive OLP group compared to the non-erosive OLP group emphasized the possible roles of HSPs in the pathogenesis and premalignant changes of OLP lesions.
近期的研究重点探讨了热休克蛋白（HSPs）等分子伴侣在扁平苔藓（LP）发病及发病机制中的可能作用。本研究首次通过实时（RT）-PCR检测，确定皮肤型扁平苔藓（CLP）和口腔型扁平苔藓（OLP）病变中 和 基因的表达情况，并与正常健康病例进行比较，同时比较OLP病变不同亚型之间的差异。
从伊朗马什哈德医科大学选取了包括56例OLP和56例CLP样本的LP病变石蜡块。还选取了56例健康正常参与者的活检样本。从他们的病历中提取人口统计学和临床特征。采用实时RT-PCR方法评估 和HPS70基因的表达。
CLP和OLP患者中 和 基因表达的比较显示，与正常参与者相比，两组中 和 基因均显著过表达（ = 0.001）。CLP和OLP患者组中 和 基因的表达均较高，但两组之间的量无显著差异。比较OLP病变的两个黏膜亚组（非糜烂性和糜烂性）显示，OLP糜烂性亚型中 和 的表达显著高于非糜烂性亚型（ = 0.001）。
与健康活检相比，LP病变中 和 过表达，我们得出结论， 和 可能在OLP和CLP病变的病因发病机制中起关键作用。与非糜烂性OLP组相比，糜烂性OLP组中 和 均过表达，这强调了HSPs在OLP病变发病机制和癌前变化中的可能作用。
