通过短期给予潜在益生菌开菲尔酵母A4和A5对BALB/c小鼠肠道微生物群进行调节
Gut microbiota modulation via short-term administration of potential probiotic kefir yeast A4 and A5 in BALB/c mice.
作者信息
Youn Hye-Young, Kim Hyeon-Jin, Kim Dong-Hyeon, Jang Yong-Seok, Kim Hyunsook, Seo Kun-Ho
机构信息
Center for One Health, Department of Veterinary Public Health, College of Veterinary Medicine, Konkuk University, Seoul, 05029 Republic of Korea.
Department of Food & Nutrition, College of Human Ecology, Hanyang University, Seoul, 04763 Republic of Korea.
出版信息
Food Sci Biotechnol. 2023 Feb 14;32(4):589-598. doi: 10.1007/s10068-023-01268-3. eCollection 2023 Mar.
UNLABELLED
Kefir yeast, , has been evaluated for its potential probiotic properties-survivability, non-pathogenicity, and antioxidant and anti-microbial activities. However, host gut microbiota modulation of kefir yeasts remains unclear. Here, we compared kefir yeast strains . A4 (Km A4) and A5 (Km A5) with ATCC MYA-796 (Sb MYA-796) by investigating their adherence to colorectal adenocarcinoma (Caco-2) cells and gut microbiota modulation in BALB/c mice. The kefir yeast strains exhibited higher intestinal cell adhesion than Sb MYA-796 ( < 0.05). Bacteroidetes, Bacteroidales, and were more abundant in the 1 × 10 CFU/mL of Km A4 treatment group than in the control group ( < 0.05). Moreover, 1 × 10 CFU/mL of Km A5 increased Corynebacteriales and compared to the 1 × 10 CFU/mL of Km A4 treatment group ( < 0.01). The results showed that Km A4 and Km A5 had good Caco-2 cell adhesion ability and modulated gut microbiota upon short-term administration in healthy mice.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-023-01268-3.
未标记
开菲尔酵母已被评估其潜在的益生菌特性——生存能力、非致病性以及抗氧化和抗菌活性。然而，开菲尔酵母对宿主肠道微生物群的调节作用仍不清楚。在此，我们通过研究开菲尔酵母菌株A4（Km A4）和A5（Km A5）对结直肠癌（Caco-2）细胞的黏附以及在BALB/c小鼠中对肠道微生物群的调节作用，将它们与酿酒酵母ATCC MYA-796（Sb MYA-796）进行了比较。开菲尔酵母菌株表现出比Sb MYA-796更高的肠道细胞黏附能力（P<0.05）。在Km A4处理组1×10⁸CFU/mL时，拟杆菌门、拟杆菌目和普雷沃氏菌属比对照组更丰富（P<0.05）。此外，与Km A4处理组1×10⁸CFU/mL相比，Km A5处理组1×10⁸CFU/mL增加了棒状杆菌目和双歧杆菌属（P<0.01）。结果表明，Km A4和Km A5在健康小鼠短期给药后具有良好的Caco-2细胞黏附能力并能调节肠道微生物群。
补充信息
在线版本包含可在10.1007/s10068-023-01268-3获取的补充材料。
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