Meral-Aktaş Hacer, Çetin Bülent, Güler Muhammet Akif, Albayrak Bülent, Tekgündüz Kadir Şerafettin, Kara Mustafa, Işlek Ali
Department of Food Engineering, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey.
Division of Pediatric Nephrology, Department of Pediatrics, Faculty of Medicine, Atatürk University, Erzurum, Turkey.
Antonie Van Leeuwenhoek. 2025 May 15;118(6):79. doi: 10.1007/s10482-025-02090-z.
The isolation and identification of probiotic yeasts is increasing rapidly. In this context, the present study aimed to isolate and identify yeast strains from neonatal faeces in Erzurum province, Türkiye and to determine their probiotic characteristics. A total of 12 yeast strains were isolated and genotypic characterization revealed the presence of seven different species, including Kluyveromyces marxianus, Candida spp. Clavispora lusitaniae, Geotrichum candidum, Trichophyton rubrum, Pichia cactophila, and Meyerozyma guilliermondii. The non-pathogenic and potentially probiotic characteristics of the K. marxianus M2, M9, and M10 strains were further investigated. Although yeast has been isolated from neonatal faeces before, K. marxianus was isolated for the first time in this study. The results revealed that the K. marxianus strains exhibited high resistance to simulated gastric juice and bile salts. The auto-aggregation percentages of the strains ranged from 92.55 to 94.78% after 4 h, while the co-aggregation percentages with pathogens ranged from 19.70 to 53.09%. The K. marxianus M2 strain exhibited the highest degree of hydrophobicity (74.97%), and none of the strains demonstrated DN-ase or haemolytic activity. Furthermore, M2 and M9 strains displayed bile salt hydrolase activity. In conclusion, based on in vitro probiotic test results, K. marxianus strains were selected as probiotic yeast candidates for further studies, especially in patients under antibiotic therapy.
益生菌酵母的分离与鉴定正在迅速增加。在此背景下，本研究旨在从土耳其埃尔祖鲁姆省的新生儿粪便中分离和鉴定酵母菌株，并确定其益生菌特性。共分离出12株酵母菌株，基因型鉴定显示存在7种不同的物种，包括马克斯克鲁维酵母、念珠菌属、葡萄牙棒孢酵母、白地霉、红色毛癣菌、仙人掌毕赤酵母和季也蒙毕赤酵母。进一步研究了马克斯克鲁维酵母M2、M9和M10菌株的非致病性和潜在的益生菌特性。虽然之前已从新生儿粪便中分离出酵母，但本研究中首次分离出马克斯克鲁维酵母。结果显示，马克斯克鲁维酵母菌株对模拟胃液和胆汁盐表现出高抗性。4小时后，这些菌株的自聚集百分比在92.55%至94.78%之间，而与病原体的共聚集百分比在19.70%至53.09%之间。马克斯克鲁维酵母M2菌株表现出最高程度的疏水性（74.97%），且所有菌株均未表现出DN酶或溶血活性。此外，M2和M9菌株表现出胆汁盐水解酶活性。总之，基于体外益生菌测试结果，马克斯克鲁维酵母菌株被选为益生菌酵母候选菌株，用于进一步研究，尤其是在接受抗生素治疗的患者中。
