Peter MacCallum Cancer Centre, Melbourne, VIC 3000, Australia.
Sir Peter MacCallum Department of Oncology, The University of Melbourne, Melbourne, VIC 3010, Australia.
STAR Protoc. 2022 Mar 9;3(1):101230. doi: 10.1016/j.xpro.2022.101230. eCollection 2022 Mar 18.
has become a popular model for examining the metabolic wasting syndrome, cachexia, characterized by degradation of muscles and fat. Here we present a protocol for quick and consistent scoring of muscle detachment, fat body lipid droplet size, and extracellular matrix (ECM) quantifications in larvae. We detail the procedures for dissecting, staining, and imaging third instar larval muscle fillets and fat body, and how to utilize FIJI macros for robust quantification of cachectic phenotypes in these dissected tissues. For complete details on the use and execution of this protocol, please refer to Lodge et al. (2021).
已成为研究代谢消耗综合征（恶病质）的流行模型，恶病质的特征是肌肉和脂肪降解。在这里，我们提出了一种在幼虫中快速且一致地评分肌肉分离、脂肪体脂肪滴大小和细胞外基质（ECM）定量的方案。我们详细介绍了解剖、染色和成像第三龄幼虫肌肉鱼片和脂肪体的过程，以及如何利用 FIJI 宏来对这些解剖组织中的恶病质表型进行稳健的定量。有关此方案的使用和执行的完整详细信息，请参阅 Lodge 等人。（2021 年）。
