迷迭香酸对 PM2.5 暴露后变应性鼻炎大鼠模型炎症反应的影响。
Effects of rosmarinic acid on the inflammatory response in allergic rhinitis rat models after PM2.5 exposure.
机构信息
Department of Otolaryngology, Huadong Hospital Affiliated to Fudan University, Shanghai, China.
Department of Environmental Science and Engineering, Center for Atmospheric Chemistry Study, Fudan University, Shanghai, China.
出版信息
J Clin Lab Anal. 2022 Apr;36(4):e24316. doi: 10.1002/jcla.24316. Epub 2022 Mar 13.
BACKGROUND
Studies have shown the promising prospects of rosmarinic acid (RosA) for the prevention and treatment of allergic diseases.
OBJECTIVE
The aim of this study was to investigate the effects of RosA on inflammatory reaction in rat models of allergic rhinitis (AR) after PM2.5 exposure.
METHODS
Allergic rhinitis rat models were established by ovalbumin sensitization, and PM2.5 was applied at a concentration of 1000 μg/m , 3 h a day for 30 consecutive days. RosA was administered via intraperitoneal injection (20 mg/kg/d) for seven consecutive days. Allergic nasal symptoms were recorded. The expressions of interleukin (IL)-4, IL-13, interferon (INF)-γ, and OVA-sIgE were determined by ELISA. Histopathological changes in nasal mucosa were observed by HE staining. mRNA expressions of T-bet and GATA-3 in nasal mucosa were detected by RT-PCR. NF-κBp65 in cell nuclei and IκBα in cytoplasm were analyzed by Western blot.
RESULTS
PM2.5 exposure worsened allergic nasal symptoms in AR rats, while RosA ameliorated these symptoms. Histopathologically, AR rats exhibited disorganized nasal mucosal epithelium, cell exfoliation, eosinophilic infiltration of lamina propria, gland swelling, and submucosal vascular congestion, which were aggravated by PM2.5 exposure and alleviated by RosA. RosA decreased the expressions of IL-4, IL-13, and increased the level of IFN-γ in PM2.5-exposed AR rats. After RosA intervention, the expressions of GATA-3 mRNA and NF-κBp65 in PM2.5-exposed AR rats were significantly reduced, while those of T-bet mRNA and IκBα were markedly increased.
CONCLUSION
Rosmarinic acid may alleviate symptoms of AR rat models exposed to PM2.5 through the modulation of the NF-κB pathway and Th1/Th2 balance.
背景
研究表明迷迭香酸（RosA）在预防和治疗过敏性疾病方面具有广阔的前景。
目的
本研究旨在探讨 RosA 对 PM2.5 暴露后变应性鼻炎（AR）大鼠模型炎症反应的影响。
方法
采用卵清蛋白致敏法建立 AR 大鼠模型，PM2.5 浓度为 1000μg/m³，每天 3 小时，连续 30 天。RosA 通过腹腔注射（20mg/kg/d）连续 7 天给药。记录变应性鼻症状。通过 ELISA 测定白细胞介素（IL）-4、IL-13、干扰素（INF）-γ和 OVA-sIgE 的表达。通过 HE 染色观察鼻黏膜组织病理学变化。通过 RT-PCR 检测鼻黏膜中 T-bet 和 GATA-3 的 mRNA 表达。通过 Western blot 分析细胞核中的 NF-κBp65 和细胞质中的 IκBα。
结果
PM2.5 暴露使 AR 大鼠的变应性鼻症状恶化，而 RosA 则改善了这些症状。组织病理学检查显示，AR 大鼠鼻黏膜上皮排列紊乱，细胞脱落，固有层嗜酸性粒细胞浸润，腺体肿胀，黏膜下血管充血，PM2.5 暴露加重了这些变化，而 RosA 则减轻了这些变化。RosA 降低了 PM2.5 暴露的 AR 大鼠中 IL-4、IL-13 的表达，并增加了 IFN-γ的水平。在 RosA 干预后，PM2.5 暴露的 AR 大鼠中 GATA-3 mRNA 和 NF-κBp65 的表达明显减少，而 T-bet mRNA 和 IκBα的表达明显增加。
结论
Rosmarinic acid 可能通过调节 NF-κB 通路和 Th1/Th2 平衡来缓解 PM2.5 暴露的 AR 大鼠模型的症状。
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