Wang Ya-Lin, Gao Wei, Li Yue, Wang Yin-Feng
Department of Otolaryngology-Head and Neck Surgery, Anhui Provincial Hospital, No. 17, Lujiang Road, Hefei, 230001, Anhui Province, China.
Eur Arch Otorhinolaryngol. 2017 Aug;274(8):3221-3229. doi: 10.1007/s00405-017-4606-8. Epub 2017 Jun 2.
Allergic rhinitis (AR) represents a clinical health issue affecting approximately 500 million people worldwide. This study aimed to explore the effects of airborne fine particulate matter (PM) on the nasal mucosa of rats with AR. Seventy-five healthy male SD rats were included and randomly divided into the normal, model, low-concentration, middle-concentration, and high-concentration groups (15 rats each group). AR rat models were established using sensitized mixture and were stimulated using different concentrations of PM. Sneeze and nose-scratching events were observed. Automatic hematology analyzer was utilized to count white blood cells (WBCs). The serum IgE, ICAM-1, and VCAM-1 expressions, eosinophil (EOS) infiltration, and IFN-γ, IL-4, IL-5, IL-33, and TSLP expressions were detected by ELISA, HE staining, and qRT-PCR. Greater numbers of WBCs, increased IgE level, elevated levels of ICAM-1, VCAM-1, EOS, IFN-γ, IL-4, IL-5, IL-33, and TSLP in the model, low-concentration, middle-concentration, and high-concentration groups than the normal group. The same trend also exhibited in rats of the middle-concentration and high-concentration groups than that of the model and low-concentration groups. Comparisons between normal rats and AR rats indicated that AR rats exhibit remarkably higher cytokine expression levels of IFN-γ, IL-4, IL-5, TSLP, and IL-33. The study revealed that as stimulation is triggered by PM, AR rats result in increased levels of adhesion molecules and inflammatory cytokine expressions in a concentration-dependent manner. Analyses of PM as well as, its effects on AR are crucial in the continued drive for both prevention and management of the disease.
变应性鼻炎（AR）是一个影响全球约5亿人的临床健康问题。本研究旨在探讨空气中细颗粒物（PM）对AR大鼠鼻黏膜的影响。纳入75只健康雄性SD大鼠，随机分为正常组、模型组、低浓度组、中浓度组和高浓度组（每组15只）。用致敏混合物建立AR大鼠模型，并用不同浓度的PM进行刺激。观察打喷嚏和挠鼻事件。使用自动血液分析仪计数白细胞（WBC）。通过ELISA、HE染色和qRT-PCR检测血清IgE、ICAM-1和VCAM-1表达、嗜酸性粒细胞（EOS）浸润以及IFN-γ、IL-4、IL-5、IL-33和TSLP表达。模型组、低浓度组、中浓度组和高浓度组的WBC数量更多，IgE水平升高，ICAM-1、VCAM-1、EOS、IFN-γ、IL-4、IL-5、IL-33和TSLP水平高于正常组。中浓度组和高浓度组大鼠也呈现出比模型组和低浓度组相同的趋势。正常大鼠与AR大鼠的比较表明，AR大鼠的IFN-γ、IL-4、IL-5、TSLP和IL-33细胞因子表达水平显著更高。研究表明，由于PM引发刺激，AR大鼠导致黏附分子水平和炎症细胞因子表达以浓度依赖性方式增加。分析PM及其对AR的影响对于该疾病的持续预防和管理至关重要。
