Department of Life Sciences and Institute of Genome Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.
Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan.
Proc Natl Acad Sci U S A. 2022 Mar 22;119(12):e2111283119. doi: 10.1073/pnas.2111283119. Epub 2022 Mar 14.
SignificanceThe adult mushroom body (MB) is one of the most extensively studied neural circuits. However, how its circuit organization is established during development is unclear. In this study, we provide an initial characterization of the assembly process of the extrinsic neurons (dopaminergic neurons and MB output neurons) that target the vertical MB lobes. We probe the cellular mechanisms guiding the neurite targeting of these extrinsic neurons and demonstrate that Semaphorin 1a is required in several MB output neurons for their dendritic innervations to three specific MB lobe zones. Our study reveals several intriguing molecular and cellular principles governing assembly of the MB circuit.
意义
成年蘑菇体（MB）是研究最广泛的神经回路之一。然而，其在发育过程中的回路组织如何建立尚不清楚。在这项研究中，我们对靶向垂直 MB 叶的外生神经元（多巴胺能神经元和 MB 输出神经元）的组装过程进行了初步描述。我们研究了指导这些外生神经元的轴突靶向的细胞机制，并证明 Semaforin 1a 对于几个 MB 输出神经元向三个特定的 MB 叶区的树突支配是必需的。我们的研究揭示了几个有趣的分子和细胞原则，这些原则控制着 MB 回路的组装。
