基于生物发光指示剂 Nano-lantern（cAMP）的 D. discoideum 中 cAMP 信号的实时成像
Live Imaging of cAMP Signaling in D. discoideum Based on a Bioluminescent Indicator, Nano-lantern (cAMP).
机构信息
Department of Optical Imaging, Advanced Research Promotion Center, Tokushima University, Tokushima City, Tokushima, Japan.
Department of Biomolecular Science and Engineering, SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Ibaraki, Osaka, Japan.
出版信息
Methods Mol Biol. 2022;2483:231-240. doi: 10.1007/978-1-0716-2245-2_14.
Bioluminescence imaging of cellular function is a promising strategy. It has advantages over fluorescence imaging such as high sensitivity, no phototoxicity or no autofluorescence, and compatibility to deep-tissue imaging or optogenetics. However, functional imaging of cellular signaling by bioluminescence is not so easy due to the limited availability of bright bioluminescent indicators.Here we describe a detailed strategy to detect cellular cAMP dynamics by using Nano-lantern (cAMP1.6), one of the brightest bioluminescent indicator for cAMP . Both induced and spontaneous cAMP signaling in social amoeba, with a large and small signal change, respectively, were imaged by this method.
细胞功能的生物发光成像是一种很有前途的策略。与荧光成像相比，它具有高灵敏度、无光毒性或自发荧光、以及与深层组织成像或光遗传学兼容等优点。然而，由于可用的明亮生物发光指示剂有限，因此通过生物发光对细胞信号进行功能成像并不那么容易。在这里，我们描述了一种使用 Nano-lantern（cAMP1.6）检测细胞 cAMP 动力学的详细策略，Nano-lantern 是最亮的 cAMP 生物发光指示剂之一。通过这种方法可以分别对具有较大和较小信号变化的社会性阿米巴中的诱导和自发的 cAMP 信号进行成像。
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