Shanmugam Balu Kolathupalayam, Rangaraj Suriyaprabha, Subramani Karthik, Srinivasan Surendhiran, Kandhasamy Narthana, Arumugam Karthik, Periyasamy Manojkumar, Aicher Wilhelm K, Venkatachalam Rajendran
Centre for Nano Science and Technology, K. S. Rangasamy College of Technology, Tiruchengode, Tamil Nadu, India.
Department of Biotechnology, Sona College of Arts and Science, Salem, Tamil Nadu, India.
J Biomed Mater Res B Appl Biomater. 2022 Aug;110(8):1942-1955. doi: 10.1002/jbm.b.35052. Epub 2022 Mar 15.
Nanostructured materials possess unique structural and functional properties that play a crucial position in tissue engineering applications. Present investigation is aimed to synthesize chitosan-sodium alginate (CS) nanocomposite using hydrothermally prepared zirconia nanoparticles. In this, three different weight percentages of (0.5, 1, and 1.5) zirconia nanoparticles are utilized for the preparation of biomimetic nanocomposite scaffolds (CSZ) employing 4 wt% of CS by a solvent casting technique. Physico-chemical and thermal behavior of the prepared nanoparticles and their CSZ scaffolds are comprehensively characterized. Bioactivity of the prepared zirconia nanoparticles and CSZ scaffolds are explored in terms of in vitro biocompatibility, protein absorption in simulated body fluid (SBF), and phosphate buffered saline (PBS). Agar disc diffusion method is employed to identify the antibacterial property against Staphylococcus aureus and Escherichia coli. In vitro cytotoxicity of zirconia nanoparticles and CSZ scaffolds is identified against human urothelial carcinoma (UC6) and osteosarcoma (MG-63) cells. These studies explore that zirconia nanoparticles are suitable for biomedical applications while it is interacted with chitosan and sodium alginate (CS) due to their promising biocompatibility. Biomimetically obtained chitosan/sodium alginate scaffold contain 1 wt% zirconia nanoparticles show higher biocompatibility amenable for tissue engineering applications.
纳米结构材料具有独特的结构和功能特性，在组织工程应用中发挥着关键作用。目前的研究旨在使用水热法制备的氧化锆纳米颗粒合成壳聚糖-海藻酸钠（CS）纳米复合材料。在此过程中，通过溶剂浇铸技术，利用三种不同重量百分比（0.5%、1%和1.5%）的氧化锆纳米颗粒，与4 wt%的CS一起制备仿生纳米复合支架（CSZ）。对制备的纳米颗粒及其CSZ支架的物理化学和热行为进行了全面表征。从体外生物相容性、在模拟体液（SBF）和磷酸盐缓冲盐水（PBS）中的蛋白质吸附方面，探索了制备的氧化锆纳米颗粒和CSZ支架的生物活性。采用琼脂圆盘扩散法鉴定对金黄色葡萄球菌和大肠杆菌的抗菌性能。针对人尿路上皮癌（UC6）和骨肉瘤（MG-63）细胞，鉴定了氧化锆纳米颗粒和CSZ支架的体外细胞毒性。这些研究表明，氧化锆纳米颗粒因其良好的生物相容性，在与壳聚糖和海藻酸钠（CS）相互作用时适用于生物医学应用。仿生获得的含1 wt%氧化锆纳米颗粒的壳聚糖/海藻酸钠支架显示出更高的生物相容性，适用于组织工程应用。
