Amrita Centre for Nanosciences, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, India.
Int J Biol Macromol. 2011 Oct 1;49(3):274-80. doi: 10.1016/j.ijbiomac.2011.04.020. Epub 2011 May 7.
The urge to repair and regenerate natural tissues can now be satisfactorily fulfilled by various tissue engineering approaches. Chitin and chitosan are the most widely accepted biodegradable and biocompatible materials subsequent to cellulose. The incorporation of nano ZrO(2) onto the chitin-chitosan scaffold is thought to enhance osteogenesis. Hence a nanocomposite scaffold was fabricated by lyophilization technique using chitin-chitosan with nano ZrO(2). The prepared nanocomposite scaffolds were characterized using SEM, FTIR, XRD and TGA. In addition, the swelling, degradation, biomineralization, cell viability and cell attachment of the composite scaffolds were also evaluated. The results demonstrated better swelling and controlled degradation in comparison to the control scaffold. Cell viability studies proved the non toxic nature of the nanocomposite scaffolds. Cells were found to be attached to the pore walls and spread uniformly throughout the scaffolds. All these results suggested that the developed nanocomposite scaffolds possess the prerequisites for tissue engineering scaffolds and could be used for various tissue engineering applications.
现在，各种组织工程方法可以令人满意地满足修复和再生天然组织的需求。壳聚糖和壳聚糖是继纤维素之后最广泛接受的可生物降解和生物相容性材料。将纳米 ZrO(2)掺入壳聚糖-壳聚糖支架被认为可以增强成骨作用。因此，采用冷冻干燥技术制备了一种纳米复合材料支架，其中使用了纳米 ZrO(2)的壳聚糖-壳聚糖。使用 SEM、FTIR、XRD 和 TGA 对制备的纳米复合材料支架进行了表征。此外，还评估了复合材料支架的溶胀、降解、生物矿化、细胞活力和细胞附着。结果表明，与对照支架相比，该支架具有更好的溶胀和控制降解性能。细胞活力研究证明了纳米复合材料支架的非毒性。发现细胞附着在孔壁上，并均匀分布在整个支架中。所有这些结果表明，所开发的纳米复合材料支架具有组织工程支架的先决条件，可用于各种组织工程应用。
