Centre for Nano Science and Technology, K. S. Rangasamy College of Technology, Tiruchengode 637215, Tamil Nadu, India.
Department of Biotechnoloy, Dr. N. G. P. Arts and Science College, Coimbatore 641048, Tamil Nadu, India.
Mater Sci Eng C Mater Biol Appl. 2020 May;110:110710. doi: 10.1016/j.msec.2020.110710. Epub 2020 Jan 31.
The study is aimed to synthesize potent metal oxide based biomimetic nanocomposites to overcome the risk associated with artificial bone tissue engineering. High purity TiO nanoparticles are synthesized via hydrothermal route. A biomimetic nanocomposite scaffolds containing chitosan-sodium alginate (4: 4) blended with three different (0.5, 1, and 1.5 wt%) concentrations of hydrothermally synthesized TiO nanoparticles are obtained by solvent casting technique. The physico-chemical and thermal degradation properties of as-synthesized TiO nanoparticles and their nanocomposite scaffolds are analyzed. In-vitro cytotoxicity and biocompatibility of the prepared TiO nanoparticles and nanocomposites are tested against human bladder tumor (UC6) and osteosarcoma (MG-63) cell lines. Antibacterial property is tested against Escherichia coli and Staphylococcus aureus. These studies reveal that TiO nanoparticles and polymeric nanocomposites contain good physico-chemical and mechanical properties for enhanced in-vitro biocompatibility suitable for biomedical applications. Biomimetically prepared chitosan-sodium alginate scaffold containing TiO nanoparticles (1 wt%) is found to exhibit superior biocompatibility for bone tissue engineering applications.
本研究旨在合成有效的基于金属氧化物的仿生纳米复合材料，以克服人工骨组织工程相关的风险。通过水热法合成高纯 TiO 纳米粒子。通过溶剂浇铸技术获得了含有壳聚糖-海藻酸钠（4：4）并掺入三种不同（0.5、1 和 1.5wt％）浓度的水热合成 TiO 纳米粒子的仿生纳米复合材料支架。分析了合成的 TiO 纳米粒子及其纳米复合材料支架的物理化学和热降解特性。针对人膀胱肿瘤（UC6）和骨肉瘤（MG-63）细胞系，对制备的 TiO 纳米粒子和纳米复合材料的体外细胞毒性和生物相容性进行了测试。针对大肠杆菌和金黄色葡萄球菌测试了抗菌性能。这些研究表明，TiO 纳米粒子和聚合物纳米复合材料具有良好的物理化学和机械性能，可提高体外生物相容性，适用于生物医学应用。含有 TiO 纳米粒子（1wt％）的仿生制备的壳聚糖-海藻酸钠支架被发现对骨组织工程应用具有优异的生物相容性。
