类风湿关节炎患者中的HOTAIR和THRIL长链非编码RNA及其靶基因
HOTAIR and THRIL Long Non Coding RNAs and Their Target Genes in Rheumatoid Arthritis patients.
作者信息
Medhat Engy, Ayeldeen Ghada, Hosni Ahmed Hanan, Shaker Olfat, Gheita Tamer, Salama Ashour Sara
机构信息
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University.
Rheumatology Department, Faculty of Medicine, Cairo University.
出版信息
Rep Biochem Mol Biol. 2022 Jan;10(4):614-621. doi: 10.52547/rbmb.10.4.697.
BACKGROUND
Rheumatoid arthtritis (RA) is a chronic systemic inflammatory autoimmune disease characterized by irreversible joint damage and deformity. The aim of this study is to investigate THRIL and HOTAIR serum expression and their target genes in Egyptian RA patients and to evaluate their relationship to the clinico-pathological data.
METHODS
The present study included fifty-two RA patients and fifty-six healthy controls. RA patients were classified according to DAS28 score. All subjects were subjected to full history taking and clinical examination. Quantitative real time PCR was done to estimate the expression levels of serum THRIL and HOTAIR as well as their target genes tumor necrosis factor alpha (TNF-α) and metalloproteinase 2 (MMP-2) were estimated by ELISA techniques.
RESULTS
Results revealed that both THRIL and HOTAIR were statistically over expressed in RA patients compared to healthy group with p-value< 0.05. Results showed as well that the target genes for those long-non coding RNAs, TNF-α and MMP-2, were also significantly higher in RA patients compared to healthy controls.
CONCLUSION
Both THRIL and HOTAIR associated with their target genes, can be considered as diagnostic markers for RA.
背景
类风湿性关节炎（RA）是一种慢性全身性炎症性自身免疫性疾病，其特征为不可逆转的关节损伤和畸形。本研究旨在调查埃及RA患者中THRIL和HOTAIR的血清表达及其靶基因，并评估它们与临床病理数据的关系。
方法
本研究纳入了52例RA患者和56例健康对照。RA患者根据DAS28评分进行分类。所有受试者均进行了全面的病史采集和临床检查。采用定量实时PCR技术估计血清THRIL和HOTAIR的表达水平，并通过ELISA技术估计其靶基因肿瘤坏死因子α（TNF-α）和金属蛋白酶2（MMP-2）的水平。
结果
结果显示，与健康组相比，RA患者中THRIL和HOTAIR在统计学上均过度表达，p值<0.05。结果还表明，与健康对照相比，这些长链非编码RNA的靶基因TNF-α和MMP-2在RA患者中也显著升高。
结论
与靶基因相关的THRIL和HOTAIR均可被视为RA的诊断标志物。
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