Cabrera Z, Cooper M D, Parkhouse R M
Trop Med Parasitol. 1986 Jun;37(2):113-6.
Adult Onchocerca gibsoni worms were fractionated into a surface-enriched fraction, a saline extract, a saline insoluble-detergent soluble fraction and a total glycoproteins extract. The antigens in each fractions were separated by electrophoresis in polyacrylamide gels and examined with an immuno-blot technique for reactive antibodies in sera from individuals infected with a variety of filarial and non-filarial nematode worms. Radiolabelled monoclonal antibodies were used to determine the Ig heavy chain isotypes. A number of antigens were demonstrated in all of the extracts, with many antigens of each extract being unique. Although some Onchocerca antigens stimulated antibodies of all human immunoglobulin classes, the panel of antigens recognized by each Ig isotype was different. The IgE response was restricted and directed at antigens not recognized by antibodies to other nematode parasites. IgM and IgA responses tended to recognize many antigens, whilst IgG responses were directed at intermediate numbers of antigens. The control of isotype balance to individual parasite antigens is thus independently regulated. This survey provides a rational basis for the exploration of Onchocerca antigen-human antibody class systems with relevance for diagnosis, protection and pathology.
将成年吉氏盘尾丝虫分为表面富集组分、盐水提取物、盐水不溶性-去污剂可溶性组分和总糖蛋白提取物。通过聚丙烯酰胺凝胶电泳分离各组分中的抗原，并用免疫印迹技术检测感染各种丝虫和非丝虫线虫的个体血清中的反应性抗体。使用放射性标记的单克隆抗体来确定Ig重链同种型。在所有提取物中都证实了多种抗原，每种提取物中的许多抗原都是独特的。虽然一些盘尾丝虫抗原能刺激所有人类免疫球蛋白类别的抗体，但每种Ig同种型识别的抗原组不同。IgE反应受到限制，且针对其他线虫寄生虫抗体未识别的抗原。IgM和IgA反应倾向于识别多种抗原，而IgG反应针对的抗原数量居中。因此，针对个体寄生虫抗原的同种型平衡控制是独立调节的。这项调查为探索与诊断、保护和病理学相关的盘尾丝虫抗原-人类抗体类别系统提供了合理依据。
