Aroonrangsee Thanwarat, Chairatnathrongporn Rachanin, Surarit Rudee, Tansriratanawong Kallapat, Santiprabhob Jeerunda, Boriboonhirunsarn Chatkoew, Promsudthi Ananya
Residency Training Programs in Periodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Private Practice, Bangkok, Thailand.
J Int Soc Prev Community Dent. 2023 Aug 30;13(4):333-341. doi: 10.4103/jispcd.JISPCD_38_23. eCollection 2023 Jul-Aug.
The primary objectives of this study were to compare salivary oxidative stress (OS) biomarker levels in patients with type 1 diabetes mellitus (T1DM) and without T1DM (non-T1DM) and evaluate the relationships between diabetes, periodontal status, and OS biomarker levels.
Twenty patients with T1DM and 20 age-matched patients without T1DM were enrolled. All participants were 15-23 years of age and had permanent dentition. Unstimulated whole saliva was collected in a sterile test tube before examination of clinical periodontal parameters, including bleeding on probing (BOP). Salivary levels of OS biomarkers-malondialdehyde, protein carbonyl, total oxidant status (TOS), and total antioxidant capacity-were determined using oxidative and antioxidative assays followed by spectrophotometric measurement at 375-532 nm. The relationships between diabetes, periodontal status, and OS biomarkers were analyzed using multiple linear regression.
TOS was significantly lower in the T1DM group compared with the non-T1DM group (5.06 ± 0.39 vs. 6.44 ± 0.51 µmol HO Eq/l, = 0.035). After adjusting for confounding factors (age, gender, BMI, clinical periodontal parameters, BOP, or diabetes status accordingly), the multiple linear regression showed that T1DM was significantly associated with a reduction of TOS level ( = 0.008). The BOP > 30% group showed a significant correlation with increased TOS levels compared with the BOP ≤ 30% group ( = 0.002). No relationship was found between OS biomarkers and HbA1c levels.
Salivary TOS levels were related to both diabetes status and the extent of gingival inflammation. Further studies to elucidate the role of OS in relation of periodontal disease and T1DM are required.
本研究的主要目的是比较1型糖尿病（T1DM）患者和非T1DM患者唾液氧化应激（OS）生物标志物水平，并评估糖尿病、牙周状况和OS生物标志物水平之间的关系。
招募20例T1DM患者和20例年龄匹配的非T1DM患者。所有参与者年龄在15 - 23岁之间，均为恒牙列。在检查包括探诊出血（BOP）在内的临床牙周参数之前，于无菌试管中收集非刺激性全唾液。使用氧化和抗氧化测定法，随后在375 - 532nm处进行分光光度测量，测定唾液中OS生物标志物——丙二醛、蛋白质羰基、总氧化剂状态（TOS）和总抗氧化能力的水平。使用多元线性回归分析糖尿病、牙周状况和OS生物标志物之间的关系。
T1DM组的TOS显著低于非T1DM组（5.06±0.39 vs. 6.44±0.51µmol HO Eq/l，P = 0.035）。在调整混杂因素（年龄、性别、BMI、临床牙周参数、BOP或糖尿病状态）后，多元线性回归显示T1DM与TOS水平降低显著相关（P = 0.008）。与BOP≤30%组相比，BOP>30%组与TOS水平升高显著相关（P = 0.002）。未发现OS生物标志物与糖化血红蛋白水平之间存在关联。
唾液TOS水平与糖尿病状态和牙龈炎症程度均相关。需要进一步研究以阐明OS在牙周疾病和T1DM关系中的作用。
