Staber F G, Tarcsay L, Dukor P
Infect Immun. 1978 Apr;20(1):40-9. doi: 10.1128/iai.20.1.40-49.1978.
Modulation of myelopoiesis by chemically pure preparations of different cell wall components from gram-negative bacteria was investigated in vivo. The effects of lipid A, outer membrane lipoprotein, and murein were evaluated at several distinct stages: induction of colony-stimulating activity (CSA) in the serum, increase in the number of committed splenic precursor cells (CFU-C) forming granulocyte-macrophage colonies in vitro, and triggering into the cell cycle of noncommitted hemopoietic stem cells (CFU-S) from bone marrow. The results reveal different patterns of activity of the bacterial cell wall components (BCWC) tested. (i) In C57Bl/6 mice and C3H/Bom mice, all three preparations were potent inducers of CSA. In C3H/HeJ mice, CSA was only induced by lipoprotein and murein and not by lipid A. After injection of lipid A or lipoprotein, but not murein, the number of CFU-C in spleens of C57Bl/6 mice was increased up to 100-fold. In C3H/Bom and C3H/HeJ mice, not only murein but also lipoprotein were much less potent in this respect. (iii) In C57Bl/6 mice, both lipid A and lipoprotein, but not murein, were capable of inducing the proliferation of CFU-S, as demonstrated by a hot thymidine cytocide technique. Thus, induction of CSA and changes in the pool size of splenic CFU-C after administration of BCWC may be unrelated events. On the other hand, the increase of CFU-C might reflect the mitogenicity of BCWC for CFU-S.
在体内研究了革兰氏阴性菌不同细胞壁成分的化学纯制剂对骨髓生成的调节作用。在几个不同阶段评估了脂多糖、外膜脂蛋白和胞壁质的作用：血清中集落刺激活性（CSA）的诱导、体外形成粒细胞-巨噬细胞集落的脾定向祖细胞（CFU-C）数量的增加，以及骨髓中未定向造血干细胞（CFU-S）进入细胞周期的触发。结果揭示了所测试的细菌细胞壁成分（BCWC）的不同活性模式。（i）在C57Bl/6小鼠和C3H/Bom小鼠中，所有三种制剂都是CSA的有效诱导剂。在C3H/HeJ小鼠中，CSA仅由脂蛋白和胞壁质诱导，而不由脂多糖诱导。注射脂多糖或脂蛋白后，但不是胞壁质，C57Bl/6小鼠脾脏中的CFU-C数量增加了100倍。在C3H/Bom和C3H/HeJ小鼠中，在这方面不仅胞壁质，而且脂蛋白的效力也低得多。（iii）在C57Bl/6小鼠中，如热胸腺嘧啶核苷细胞杀伤技术所示，脂多糖和脂蛋白都能够诱导CFU-S的增殖，而胞壁质则不能。因此，BCWC给药后CSA的诱导和脾CFU-C池大小的变化可能是不相关的事件。另一方面，CFU-C的增加可能反映了BCWC对CFU-S的促有丝分裂作用。
