Mercader Anthony, Ye Sang-Ho, Kim Seungil, Orizondo Ryan A, Cho Sung Kwon, Wagner William R
Department of Mechanical Engineering & Materials Science, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States.
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, United States.
Langmuir. 2022 Mar 29;38(12):3775-3784. doi: 10.1021/acs.langmuir.1c03375. Epub 2022 Mar 16.
Poly(dimethylsiloxane) (PDMS) has been used in a wide range of biomedical devices and medical research due to its biostability, cytocompatibility, gas permeability, and optical properties. Yet, some properties of PDMS create critical limitations, particularly fouling through protein and cell adhesion. In this study, a diallyl-terminated sulfobetaine (SB-diallyl) molecule was synthesized and then directly mixed with a commercial PDMS base (Sylgard 184) and curing agent to produce a zwitterionic group-bearing PDMS (PDMS-SB) hybrid that does not require a complex or an additional surface modification process for the desired end product. In vitro examination of antifouling behavior following exposure to fresh ovine blood showed a significant reduction in platelet deposition for the PDMS-SB hybrid surface compared to that of a PDMS control ( < 0.05, = 5). The manufacturability via soft lithography using the synthesized polymers was found to be comparable to that for unmodified PDMS. Bonding via O plasma treatment was confirmed, and the strength was measured and again found to be comparable to the control. PDMS-SB microfluidic devices were successfully fabricated and showed improved blood compatibility that could reduce channel occlusion due to clot formation relative to PDMS control devices. Further, gas (CO) transfer through a PDMS-SB hybrid membrane was also tested with a proof-of-concept microchannel device and shown to be comparable to that through the PDMS control.
聚二甲基硅氧烷（PDMS）因其生物稳定性、细胞相容性、气体渗透性和光学特性，已被广泛应用于各种生物医学设备和医学研究中。然而，PDMS的一些特性带来了严重的限制，尤其是蛋白质和细胞粘附导致的污染问题。在本研究中，合成了一种二烯丙基封端的磺基甜菜碱（SB-二烯丙基）分子，然后将其直接与商用PDMS基体（Sylgard 184）和固化剂混合，以制备一种带有两性离子基团的PDMS（PDMS-SB）杂化物，该杂化物无需复杂或额外的表面改性工艺即可得到所需的最终产品。对暴露于新鲜羊血后的防污行为进行的体外检查表明，与PDMS对照相比，PDMS-SB杂化表面的血小板沉积显著减少（<0.05，=5）。发现使用合成聚合物通过软光刻法的可制造性与未改性的PDMS相当。通过O等离子体处理的键合得到了证实，并对强度进行了测量，结果再次表明与对照相当。成功制造了PDMS-SB微流控设备，与PDMS对照设备相比，其血液相容性得到改善，可减少由于血栓形成导致的通道阻塞。此外，还使用概念验证微通道设备测试了通过PDMS-SB杂化膜的气体（CO）传输，结果表明与通过PDMS对照的传输相当。
