Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 17579, Republic of Korea.
AceEMzyme Co., Ltd., Academic Industry Cooperation, 327 Chungang-no, Anseong-si, Kyonggi-do, 17579, Republic of Korea.
Curr Microbiol. 2022 Mar 16;79(5):134. doi: 10.1007/s00284-022-02814-8.
A novel Gram-stain-negative, aerobic, yellowish-pigmented, non-motile, rod-shaped bacterial strain, designated strain BO-59, was isolated from the activated sludge of a wastewater treatment plant in Hanam City, South Korea. Phylogenetic study based on the 16S rRNA gene sequence positioned BO-59 in a distinct lineage in the family Chitinophagaceae, sharing less than 92.8% sequence similarity with members of the closely related genera Ferruginibacter, Flavitalea, Pseudoflavitalea, Flavisolibacter, Niastella, and Terrimonas. Phylogenomic- and genomic relatedness analyses revealed that strain BO-59 is clearly distinguished from other genera in the family Chitinophagaceae by average nucleotide identity < 66.9%) and the genome-to-genome distance (< 29.5%) values. The strain BO-59 contained MK-7 as the predominant quinone, and iso-C, iso-C 3OH, and iso-C G as major fatty acids (> 10%). The DNA G + C content was 39.1 mol% based on genome sequence analysis. The polar lipids of strain BO-59 were phosphatidylethanolamine, an unidentified aminophospholipid and three unidentified polar lipids. 16S rRNA gene sequence similarity, physiological, and biochemical characteristics indicated that strain BO-59 represents a novel species of a new genus, for which the name Hanamia caeni gen. nov., sp. nov. is proposed. The type strain is BO-59 (= KACC 19646 = LMG 30865).
一株新型革兰氏阴性、需氧、黄色素、非运动、杆状细菌菌株，被命名为 BO-59 菌株，从韩国华城的一家污水处理厂的活性污泥中分离得到。基于 16S rRNA 基因序列的系统发育研究将 BO-59 定位于几丁质噬菌科的一个独特谱系中，与亲缘关系较近的 Ferruginibacter、Flavitalea、Pseudoflavitalea、Flavisolibacter、Niastella 和 Terrimonas 属成员的序列相似性小于 92.8%。系统基因组和基因组相关性分析表明，BO-59 菌株与几丁质噬菌科的其他属通过平均核苷酸同一性 (<66.9%) 和基因组到基因组的距离 (<29.5%) 值明显区分开来。菌株 BO-59 含有 MK-7 作为主要醌，以及 iso-C、iso-C 3OH 和 iso-C G 作为主要脂肪酸 (>10%)。根据基因组序列分析，菌株 BO-59 的 DNA G+C 含量为 39.1 mol%。菌株 BO-59 的极性脂为磷脂酰乙醇胺、一种未鉴定的氨基磷脂和三种未鉴定的极性脂。16S rRNA 基因序列相似性、生理和生化特征表明，菌株 BO-59 代表一个新属的新种，建议将其命名为 Hanamia caeni gen. nov.，sp. nov.。模式菌株为 BO-59(=KACC 19646=LMG 30865)。
