Gasser Benedikt, Franchi Martino V, Ruoss Severin, Frei Annika, Popp Werner L, Niederseer David, Catuogno Silvio, Frey Walter O, Flück Martin
Departement für Bewegung und Sport - Universität Basel, Basel, Switzerland.
Spinal Cord Injury Center, Balgrist University Hospital, Zurich, Switzerland.
Front Sports Act Living. 2022 Feb 28;4:814975. doi: 10.3389/fspor.2022.814975. eCollection 2022.
The insertion/deletion (I/D) polymorphism in the gene for the major regulator of vascular tone, angiotensin-converting enzyme-insertion/deletion (ACE-I/D) affects muscle capillarization and mitochondrial biogenesis with endurance training. We tested whether changes of leg muscle oxygen saturation (SmO) during exhaustive exercise and recovery would depend on the aerobic fitness status and the ACE I/D polymorphism.
In total, 34 healthy subjects (age: 31.8 ± 10.2 years, 17 male, 17 female) performed an incremental exercise test to exhaustion. SmO in (VAS) and musculus gastrocnemius (GAS) was recorded with near-IR spectroscopy. Effects of the aerobic fitness status (based on a VO cutoff value of 50 ml O min kg) and the ACE-I/D genotype (detected by PCR) on kinetic parameters of muscle deoxygenation and reoxygenation were assessed with univariate ANOVA.
Deoxygenation with exercise was comparable in VAS and GAS ( = 0.321). In both leg muscles, deoxygenation and reoxygenation were 1.5-fold higher in the fit than the unfit volunteers. Differences in muscle deoxygenation, but not VOpeak, were associated with gender-independent ( > 0.58) interaction effects between aerobic fitness × ACE-I/D genotype; being reflected in a 2-fold accelerated deoxygenation of VAS for aerobically fit than unfit ACE-II genotypes and a 2-fold higher deoxygenation of GAS for fit ACE-II genotypes than fit D-allele carriers.
Aerobically fit subjects demonstrated increased rates of leg muscle deoxygenation and reoxygenation. Together with the higher muscle deoxygenation in aerobically fit ACE-II genotypes, this suggests that an ACE-I/D genotype-based personalization of training protocols might serve to best improve aerobic performance.
血管紧张素转换酶插入/缺失(ACE-I/D)基因中的插入/缺失(I/D)多态性是血管张力的主要调节因子,它会影响耐力训练中的肌肉毛细血管化和线粒体生物合成。我们测试了力竭运动及恢复过程中腿部肌肉氧饱和度(SmO)的变化是否取决于有氧适能状态和ACE I/D多态性。
共有34名健康受试者(年龄:31.8±10.2岁,男性17名,女性17名)进行递增运动试验直至力竭。使用近红外光谱记录股直肌(VAS)和腓肠肌(GAS)中的SmO。采用单因素方差分析评估有氧适能状态(基于VO₂截止值50 ml O₂/min/kg)和ACE-I/D基因型(通过PCR检测)对肌肉脱氧和复氧动力学参数的影响。
运动时VAS和GAS中的脱氧情况相当(P = 0.321)。在两条腿部肌肉中,适能志愿者的脱氧和复氧情况比非适能志愿者高1.5倍。肌肉脱氧的差异而非峰值摄氧量(VO₂peak)与有氧适能×ACE-I/D基因型之间的性别独立(P > 0.58)交互作用有关;这体现在有氧适能的ACE-II基因型比非适能的ACE-II基因型VAS的脱氧速度快2倍,适能ACE-II基因型的GAS脱氧情况比适能D等位基因携带者高2倍。
有氧适能的受试者腿部肌肉脱氧和复氧速率增加。再加上有氧适能的ACE-II基因型中较高的肌肉脱氧情况,这表明基于ACE-I/D基因型的训练方案个性化可能最有助于提高有氧运动表现。