State Key Laboratory of Phytochemistry and Plant Resources in West China, and Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, and Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Fitoterapia. 2022 Apr;158:105174. doi: 10.1016/j.fitote.2022.105174. Epub 2022 Mar 13.
Five new cholestane glycosides, named parisfargosides A-E (1-5), were isolated from the rhizomes of Paris fargesii. Their structures were elucidated on the basis of UV, HR-ESI-MS, 1D and 2D NMR data as well as chemical methods. The structures of all compounds contained α, β-unsaturated ketone unit. Compounds 3-5 possessed a 16,23-cyclocholest skeleton with 6/6/6/5/5 condensed ring, and the absolute configurations of C-16 and C-23 were confirmed according to ROESY spectra with pyridine‑d and DMSO‑d as solvents. In addition, the platelet aggregation activity and cytotoxic activity against five human cancer cell lines (HL-60, A549, SMMC-7721, MDA-MB-231, and SW480) of compounds 1-5 were evaluated.
从武当贝母的根茎中分离得到了五个新的胆甾烷糖苷，命名为巴黎 fargosides A-E（1-5）。基于 UV、HR-ESI-MS、1D 和 2D NMR 数据以及化学方法阐明了它们的结构。所有化合物的结构都含有α，β-不饱和酮单元。化合物 3-5 具有 16,23-环胆甾骨架和 6/6/6/5/5 稠合环，根据吡啶-d 和 DMSO-d 作为溶剂的 ROESY 谱确定了 C-16 和 C-23 的绝对构型。此外，还评估了化合物 1-5 对五种人癌细胞系（HL-60、A549、SMMC-7721、MDA-MB-231 和 SW480）的血小板聚集活性和细胞毒性活性。
