Department of Molecular and Cell Biology, Faculty of Science, University of Cape Town, Cape Town, South Africa.
Institute of Infectious Disease & Molecular Medicine, University of Cape Town, Cape Town, South Africa.
PLoS One. 2022 Mar 17;17(3):e0265326. doi: 10.1371/journal.pone.0265326. eCollection 2022.
Skin colonization with coagulase-negative staphylococci (CoNS) is generally beneficial, but recent investigations suggest its association with flares and atopic dermatitis (AD) severity. However, this relationship remains unclear.
To assess patterns of staphylococcal colonization and biofilm formation in toddlers with and without AD from rural and urban South African settings.
We conducted a cross-sectional study of AD-affected and non-atopic AmaXhosa toddlers from rural Umtata and urban Cape Town, South Africa. CoNS isolates were recovered from lesional, nonlesional skin samples and the anterior nares of participants. Identification of the staphylococci was achieved by MALDI-TOF mass spectrometry. The microtiter plate assay assessed in-vitro biofilm formation.
CoNS and S. aureus commonly co-colonized nonlesional skin among cases (urban: 24% vs. 3%, p = 0.037 and rural 21% vs. 6%, p<0.001), and anterior nares in urban cases (24% vs. 0%, p = 0.002) than the control group. S. capitis colonization on nonlesional skin and anterior nares was positively associated with more severe disease in rural (48.3±10.8 vs. 39.7±11.5, P = 0.045) and urban cases (74.9±10.3 vs. 38.4±13, P = 0.004), respectively. Biofilm formation was similar between cases and controls, independent of rural-urban living.
CoNS colonization is associated with AD and disease severity and may be implicated in AD exacerbations. Studies are needed to understand their underlying pathological contribution in AD pathogenesis.
凝固酶阴性葡萄球菌（CoNS）的皮肤定植通常是有益的，但最近的研究表明，它与发作和特应性皮炎（AD）的严重程度有关。然而，这种关系尚不清楚。
评估来自南非农村和城市环境的患有和未患有 AD 的幼儿葡萄球菌定植和生物膜形成模式。
我们对来自南非农村乌姆塔塔和城市开普敦的受 AD 影响和非特应性 AmaXhosa 幼儿进行了横断面研究。从参与者的病变和非病变皮肤样本以及前鼻孔中回收 CoNS 分离株。通过 MALDI-TOF 质谱法鉴定葡萄球菌。微量滴定板测定评估体外生物膜形成。
CoNS 和金黄色葡萄球菌通常在农村病例（24%比 3%，p = 0.037）和城市病例（21%比 6%，p<0.001）的非病变皮肤上共同定植，在城市病例的前鼻孔中（24%比 0%，p = 0.002）比对照组更常见。非病变皮肤和前鼻孔的 S. capitis 定植与农村病例（48.3±10.8 比 39.7±11.5，P = 0.045）和城市病例（74.9±10.3 比 38.4±13，P = 0.004）的疾病更严重呈正相关。无论城乡居住，生物膜形成在病例和对照组之间均相似。
CoNS 定植与 AD 和疾病严重程度相关，可能与 AD 加重有关。需要研究了解它们在 AD 发病机制中的潜在病理贡献。
