Universidade Paranaense, Praça Mascarenhas de Moraes, 4282, 87502-210, Umuarama, PR, Brazil.
Universidade Federal da Bahia, Rua Augusto Viana, s/n, 40110-909, Salvador, BA, Brazil.
World J Microbiol Biotechnol. 2022 Mar 17;38(5):74. doi: 10.1007/s11274-022-03257-w.
Lentinus crinitus basidiocarps are an alternative to antimicrobials, but the stipe (24% basidiocarp) is discarded even with potential antimicrobial activity. This study evaluated the antimicrobial activity of L. crinitus basidiocarp pileus and stipe extracts against foodborne pathogens and food spoilage microorganisms. Basidiocarps of L. crinitus were grown in sugarcane bagasse and rice husks and the pileus and stipe methanolic extract was analyzed by broth microdilution method for antimicrobial activity against eight bacteria and eight fungi. The minimum bactericidal concentration values for pileus and stipe ranged from 0.40 to 0.50 mg mL, for streptomycin from 0.10 to 0.50 mg mL, and for ampicillin from 0.40 to 1.20 mg mL. The minimum fungicidal concentration values for pileus and stipe ranged from 0.06 to 0.60 mg mL, for bifonazole from 0.20 to 0.25 mg mL, and for ketoconazole from 0.30 to 3.50 mg mL. Extracts had bacteriostatic, bactericidal, fungistatic and fungicidal activity against all microorganisms, but with greater efficiency and specificity for some microorganisms. Both pileus and stipe are promising and sustainable alternatives for use in food, agricultural, and pharmaceutical industries.
毛韧革菌菌盖和菌柄提取物具有替代抗生素的潜力，但是菌柄（占菌盖的 24%）即使具有潜在的抗菌活性也会被丢弃。本研究评估了毛韧革菌菌盖和菌柄提取物对食源性致病菌和食品腐败微生物的抗菌活性。在甘蔗渣和稻壳上培养毛韧革菌菌盖和菌柄，采用肉汤微量稀释法分析菌盖和菌柄甲醇提取物的抗菌活性，检测对象为 8 种细菌和 8 种真菌。菌盖和菌柄的最低杀菌浓度值范围为 0.40-0.50mg/mL，链霉素为 0.10-0.50mg/mL，氨苄西林为 0.40-1.20mg/mL。菌盖和菌柄的最低抑菌浓度值范围为 0.06-0.60mg/mL，比伏康唑为 0.20-0.25mg/mL，酮康唑为 0.30-3.50mg/mL。提取物对所有微生物均具有抑菌、杀菌、抑菌和杀菌活性，但对某些微生物的效率和特异性更高。菌盖和菌柄均是有前途的可持续替代品，可用于食品、农业和制药行业。
