ASTA MicroIDSys 系统，一种新型基质辅助激光解吸电离飞行时间质谱系统，用于鉴定韩国参考和临床细菌及酵母菌株的准确性。
Accuracy of ASTA MicroIDSys, a new matrix-assisted laser desorption/ionization time-of-flight mass spectrometry system, for the identification of Korean reference and clinical bacterial and yeast strains.
机构信息
Department of Laboratory Medicine, College of Medicine, Chosun University, Gwangju, South Korea.
Premedical Science, Chosun University College of Medicine, Gwangju, South Korea.
出版信息
Diagn Microbiol Infect Dis. 2022 May;103(1):115658. doi: 10.1016/j.diagmicrobio.2022.115658. Epub 2022 Feb 3.
This study compared the accuracy of a new MALDI-TOF mass spectrometry system, ASTA MicroIDSys system, with that of MALDI Biotyper system for the identification of reference and clinical bacterial and yeast strains. The identification accuracy of the 2 systems was compared using a total of 406 strains comprising 142 aerobic and 180 anaerobic bacterial strains and 84 yeast strains. The genus and species identification rates were 98.0% and 89.4% using MicroIDSys and 96.1% and 89.4% using Biotyper, respectively. The species identification rates of MicroIDSys and Biotyper for aerobic bacteria were 93.0% and 97.2%, respectively, and those for anaerobic bacteria were 85.6% and 81.7%, respectively. The accuracy of yeast identification at the species level was 91.7% using MicroIDSys and 92.9% using Biotyper. These findings indicate that MicroIDSys could be useful for the accurate identification of bacteria and yeast in clinical microbiology laboratories.
本研究比较了一种新的 MALDI-TOF 质谱系统，ASTA MicroIDSys 系统，与 MALDI Biotyper 系统在鉴定参考和临床细菌和酵母菌株方面的准确性。使用总共 406 株菌株比较了这 2 个系统的鉴定准确性，其中包括 142 株需氧菌和 180 株厌氧菌以及 84 株酵母菌株。MicroIDSys 的属和种鉴定率分别为 98.0%和 89.4%，Biotyper 分别为 96.1%和 89.4%。MicroIDSys 和 Biotyper 对需氧菌的种鉴定率分别为 93.0%和 97.2%，对厌氧菌的种鉴定率分别为 85.6%和 81.7%。MicroIDSys 对酵母种水平的鉴定准确性为 91.7%，Biotyper 为 92.9%。这些发现表明 MicroIDSys 可用于临床微生物学实验室中细菌和酵母的准确鉴定。
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