Levy R A, Warford A L
Am J Clin Pathol. 1986 Sep;86(3):330-5. doi: 10.1093/ajcp/86.3.330.
The modified Chlamydiazyme enzyme immunoassay (EIA) was compared with McCoy cell culture with 336 paired genital swabs collected from patients attending gynecology and urgent care clinics and transported to the regional laboratory for testing. Of the 299 female and 37 male genital specimens, 47 (15.7%) cervical and 14 (37.8%) urethral specimens were positive. All 60 specimens positive for Chlamydia trachomatis also were EIA reactive (sensitivity, 100%). The specificity was 91.3%, with 24 paired swab specimens positive by EIA only. A number of factors may have contributed some culture false negatives, as 91.7% of the EIA only positive patients were symptomatic. The reproducibility of the EIA method was evaluated with different washing technics. The Chlamydiazyme EIA was found to be a cost-effective, rapid, and sensitive test for detection of chlamydial antigen in genital specimens.
将改良的衣原体酶免疫测定法（EIA）与 McCoy 细胞培养法进行比较，使用从妇科和紧急护理诊所就诊患者收集的 336 对生殖器拭子，并运送到区域实验室进行检测。在 299 份女性和 37 份男性生殖器标本中，47 份（15.7%）宫颈标本和 14 份（37.8%）尿道标本呈阳性。所有 60 份沙眼衣原体阳性标本的 EIA 也呈反应性（敏感性为 100%）。特异性为 91.3%，仅 24 对拭子标本 EIA 呈阳性。一些因素可能导致了一些培养假阴性，因为仅 EIA 阳性的患者中有 91.7%有症状。使用不同的洗涤技术评估了 EIA 方法的可重复性。发现衣原体酶 EIA 是一种检测生殖器标本中衣原体抗原的经济有效、快速且敏感的检测方法。
