小鼠尾部皮肤全铺片染色和活体钙成像。

Mouse tail skin wholemount staining and intravital calcium imaging.

机构信息

National Institute of Biological Sciences, Beijing, China.

Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, China.

出版信息

STAR Protoc. 2022 Mar 10;3(1):101235. doi: 10.1016/j.xpro.2022.101235. eCollection 2022 Mar 18.

DOI:10.1016/j.xpro.2022.101235

PMID:35300002

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8920931/

Abstract

Mouse tail skin offers multiple benefits for skin research. Here, we present a protocol detailing high resolution tail skin whole mount staining and calcium imaging of hair follicle stem cells (HFSCs) using and mice. This approach enables the study of hair follicles and stem cells in different physiological and pathological conditions. The applications of the protocol include visualization of calcium signaling in other cell types with Cre/CreER lines or analyzing other cellular features with different reporter lines. For complete details on the use and execution of this protocol, please refer to Xie et al. (2022).

摘要

鼠尾皮肤为皮肤研究提供了多种益处。在这里，我们提供了一个详细的方案，介绍了如何使用和小鼠进行高分辨率尾皮全铺片染色和毛囊干细胞（HFSCs）的钙成像。这种方法可以研究不同生理和病理条件下的毛囊和干细胞。该方案的应用包括使用 Cre/CreER 系观察其他细胞类型中的钙信号，或使用不同的报告基因系分析其他细胞特征。如需了解本方案的详细使用和执行情况，请参考 Xie 等人（2022 年）的研究。