CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology, Beijing, 100190, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Angew Chem Int Ed Engl. 2022 May 23;61(22):e202114706. doi: 10.1002/anie.202114706. Epub 2022 Mar 30.
Here, we describe a DNA circuit-aided, origami nanodevice-based plasmonic system, which performs DNA-regulated, cascade amplification of faint chemical/biological signals. In this system, two gold-nanorods (GNRs) are co-assembled onto a DNA lock-containing, tweezer-like DNA origami template. Logic circuits serve as recognition and amplification elements for specific messengers, producing DNA keys for driving conformational changes of the plasmonic nanodevices. In the presence of input signals including nucleic acids, adenosines, chiral tyrosinamides or specific receptors expressed by tumor cells, the plasmonic nanodevices can be activated to perform dynamic structural motions, reporting robust responses via plasmonic circular dichroism (CD) spectral changes. This DNA nanodevice-based system provides a different design to enrich the strategies for constructing synthetic nanomachines, enabling the customized bottom-up nanostructure construction for sensitive biological signaling.
在这里，我们描述了一个 DNA 电路辅助的折纸纳米器件基等离子体系统，该系统可实现微弱化学/生物信号的 DNA 调控级联放大。在该系统中，两个金纳米棒（GNRs）共组装到含有 DNA 锁的镊子状 DNA 折纸模板上。逻辑电路作为特定信使的识别和放大元件，产生用于驱动等离子体纳米器件构象变化的 DNA 键。在存在包括核酸、腺嘌呤、手性酪氨酸酰胺或肿瘤细胞表达的特定受体等输入信号的情况下，等离子体纳米器件可以被激活以执行动态结构运动，通过等离子体圆二色性 (CD) 光谱变化报告稳健的响应。这个基于 DNA 纳米器件的系统提供了一种不同的设计，丰富了构建合成纳米机器的策略，使定制的自下而上的纳米结构构建能够用于敏感的生物信号。
