Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, PR China.
Key Laboratory of Luminescent and Real-Time Analytical System (Southwest University), Chongqing Science and Technology Bureau, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.
Biosens Bioelectron. 2022 Jul 1;207:114183. doi: 10.1016/j.bios.2022.114183. Epub 2022 Mar 11.
DNA logic computing has captured increasing interest due to its ability to assemble programmable DNA computing elements for disease diagnosis, gene regulation, and targeted therapy. In this work, we developed an aptamer-equipped high-integrated DNA biocomputing platform (HIDBP-A) with a dual-recognition function that enabled cancer cell targeting. Dual microRNAs were the input signals and can perform AND logic operations. Compared to the free DNA biocomputing platform (FDBP), the integration of all computing elements into the same DNA tetrahedron greatly improved logic computing speed and efficiency owing to the confinement effect reflected by the high local concentration of computing elements. As a proof of concept, the utilization of microRNA as the input signal was beneficial for improving the scalability and flexibility of the sequence design of the logic nano-platform. Given that the different microRNAs were over-expressed in cancer cells, this new HIDBP-A has great promise in accurate diagnosis and logic-controlled disease treatment.
DNA 逻辑计算因其能够组装可编程的 DNA 计算元件，用于疾病诊断、基因调控和靶向治疗，因此引起了越来越多的关注。在这项工作中，我们开发了一种带有双重识别功能的适体装备的高集成 DNA 生物计算平台（HIDBP-A），可实现癌细胞靶向。双重 microRNA 作为输入信号，可以进行 AND 逻辑运算。与游离 DNA 生物计算平台 (FDBP) 相比，由于计算元件的高局部浓度所反映的限制效应，将所有计算元件集成到相同的 DNA 四面体中极大地提高了逻辑计算速度和效率。作为概念验证，将 microRNA 用作输入信号有利于提高逻辑纳米平台序列设计的可扩展性和灵活性。鉴于不同的 microRNA 在癌细胞中过度表达，这种新型 HIDBP-A 在准确诊断和逻辑控制疾病治疗方面具有广阔的应用前景。
