聚合酶链反应（PCR）检测环境样本中嗜肺军团菌的检测性能：系统评价和荟萃分析。
Detection performance of PCR for Legionella pneumophila in environmental samples: a systematic review and meta-analysis.
机构信息
Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
Department of Pediatrics, The Pediatrics School of Guangzhou Medical University, Guangzhou, 510182, China.
出版信息
Ann Clin Microbiol Antimicrob. 2022 Mar 18;21(1):12. doi: 10.1186/s12941-022-00503-9.
BACKGROUND
Legionellosis remains a public health problem. The most common diagnostic method to detect Legionella pneumophila (L. pneumophila) is culture. Polymerase chain reaction (PCR) is a fast and accurate method for this detection in environmental samples.
METHODS
Four databases were searched for studies that evaluated the detection efficiency of PCR in L. pneumophila. The quality evaluation was conducted using Review Manager 5.3. We used Meta-DiSc 1.4 software and the Stata 15.0 software to create forest plots, a meta-regression, a bivariate boxplot and a Deeks' funnel plot.
RESULTS
A total of 18 four-fold tables from 16 studies were analysed. The overall pooled sensitivity and specificity of PCR was 94% and 72%, respectively. The positive likelihood ratio (RLR) and negative likelihood ratio (NLR) was 2.73 and 0.12, respectively. The result of the diagnostic odds ratio (DOR) was 22.85 and the area under the curve (AUC) was 0.7884.
CONCLUSION
Establishing a laboratory diagnostic tool for L. pneumophila detection is important for epidemiological studies. In this work, PCR demonstrated a promising diagnostic accuracy for L. pneumophila.
背景
军团病仍然是一个公共卫生问题。检测嗜肺军团菌（L. pneumophila）最常用的诊断方法是培养。聚合酶链反应（PCR）是一种快速、准确的检测环境样本中军团菌的方法。
方法
在四个数据库中搜索评估 PCR 在 L. pneumophila 检测中的效率的研究。使用 Review Manager 5.3 进行质量评估。我们使用 Meta-DiSc 1.4 软件和 Stata 15.0 软件创建森林图、meta 回归、双变量箱线图和 Deeks' 漏斗图。
结果
共分析了来自 16 项研究的 18 个四格表。PCR 的总体合并敏感性和特异性分别为 94%和 72%。阳性似然比（LR）和阴性似然比（LR）分别为 2.73 和 0.12。诊断优势比（DOR）的结果为 22.85，曲线下面积（AUC）为 0.7884。
结论
建立用于检测嗜肺军团菌的实验室诊断工具对于流行病学研究很重要。在这项工作中，PCR 显示出对嗜肺军团菌具有有前景的诊断准确性。
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