College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
College of Food Science, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
Vet Res. 2022 Mar 18;53(1):21. doi: 10.1186/s13567-022-01042-z.
The aim of this study was to investigate whether oral administration of Lactobacillus brevis 23017 (LB) alone and in combination with ellagic acid inhibits ChTLR15/ChNLRP3/ChIL-1β by activating the Nrf2/HO-1 pathway to attenuate intestinal inflammatory injury. Two animal experiments were performed. In Experiment 1, chickens were allocated into 7 groups: PBS, and low, medium and high dosages of live and heat-killed LB, named L/LB(+), M/LB(+) and H/LB(+), and L/LB(-), M/LB(-) and H/LB(-), respectively. In Experiment 2, chickens were divided into 5 groups: PBS, challenge control, and low, medium and high dosages of ellagic acid combined with LB(+), named L/EA + L/LB(+), M/EA + M/LB(+) and H/EA + H/LB(+), respectively. Chickens were gavaged with LB with or without ellagic acid once a day. Then, the mRNA and protein levels of the components of the Nrf2/HO-1 pathway found in the caecal tissues were quantified. On Day 7 post-infection with E. tenella, the levels of the components of the ChTLR15/NLRP3/IL-1β pathway in the caeca were again quantified, and the anticoccidial effects were assessed. The results showed that the levels of the genes in the Nrf2/HO-1 pathway in the chickens in the LB(+) groups were higher than those in the LB(-) groups (p < 0.001); those in the H/LB(+) group were higher than those in the M/LB(+) and L/LB(+) groups (p < 0.001); and those in the H/EA + H/LB(+) group showed the highest expression levels compared with the other groups (p < 0.001). After challenge, the chickens in the H/LB(+) group displayed less inflammatory injury than those in the M/LB(+) and L/LB(+) groups (p < 0.05), and the chickens in the H/EA + H/LB(+) group showed stronger anti-inflammatory effects than the other groups (p < 0.05). Thus, these protective effects against infection were consistent with the above results. Overall, significant anti-inflammatory effects were observed in chickens orally gavaged with high dosages of live L. brevis 23017 and ellagic acid, which occurred by regulation of the ChTLR15/NLRP3/IL-1β pathway.
本研究旨在探讨单独口服短双歧杆菌 23017（LB）以及联合鞣花酸是否通过激活 Nrf2/HO-1 通路抑制 ChTLR15/ChNLRP3/ChIL-1β，从而减轻肠道炎症损伤。进行了两项动物实验。在实验 1 中，将鸡分为 7 组：PBS 组，以及低、中、高剂量活和热处理 LB 组，分别命名为 L/LB(+)、M/LB(+)和 H/LB(+)，以及 L/LB(-)、M/LB(-)和 H/LB(-)。在实验 2 中，将鸡分为 5 组：PBS 组、攻毒对照组，以及低、中、高剂量鞣花酸联合 LB(+)组，分别命名为 L/EA+L/LB(+)、M/EA+M/LB(+)和 H/EA+H/LB(+)。鸡每天灌胃一次 LB 和/或鞣花酸。然后，定量分析盲肠组织中 Nrf2/HO-1 通路成分的 mRNA 和蛋白水平。在感染柔嫩艾美耳球虫后第 7 天，再次定量分析盲肠中 ChTLR15/NLRP3/IL-1β 通路成分的水平，并评估抗球虫效果。结果表明，LB(+)组鸡的 Nrf2/HO-1 通路基因水平高于 LB(-)组（p<0.001）；H/LB(+)组高于 M/LB(+)和 L/LB(+)组（p<0.001）；H/EA+H/LB(+)组的表达水平最高（p<0.001）。攻毒后，H/LB(+)组鸡的炎症损伤程度低于 M/LB(+)和 L/LB(+)组（p<0.05），H/EA+H/LB(+)组的抗炎效果强于其他组（p<0.05）。因此，这些抗感染的保护作用与上述结果一致。总的来说，高剂量活短双歧杆菌 23017 和鞣花酸口服灌胃对鸡具有显著的抗炎作用，其作用机制与调控 ChTLR15/NLRP3/IL-1β 通路有关。
