Hanai N, Shitara K, Yoshida H
Cancer Res. 1986 Oct;46(10):5206-10.
The distribution of a variant of blood group A antigen recognized by a murine monoclonal antibody, KM-32, generated against human squamous cell lung carcinoma was investigated in various tissues and sera. By immunoperoxidase staining, the antibody was found to react with a number of lung carcinoma tissues of squamous cell carcinoma, adenocarcinoma, and small cell carcinoma, and several other tumor tissues. Positive staining was also observed in a small number of cells of some normal tissues, such as bronchiolar epithelium, gastrointestinal glands, and convoluted tubules of the kidney. The antibody could also be used in detecting macromolecular antigens, designated KA-32, in sera of patients with lung cancer. The antigen level in serum was determined by an inhibition assay using purified KM-32. The higher level of inhibition was seen in sera from over half of patients with lung cancer and patients with benign diseases when compared with those in sera from healthy adults. Purification of the antigen in serum was performed by gel filtration chromatography, immunoaffinity chromatography, and polyacrylamide gradient gel electrophoresis. Purified antigen exhibited a glycoprotein nature, and its molecular weight was estimated at more than 500,000.
研究了一种针对人肺鳞状细胞癌产生的鼠单克隆抗体KM - 32所识别的A型血型抗原变体在各种组织和血清中的分布情况。通过免疫过氧化物酶染色发现，该抗体可与多种肺癌组织发生反应，包括鳞状细胞癌、腺癌和小细胞癌，以及其他一些肿瘤组织。在一些正常组织的少数细胞中也观察到阳性染色，如细支气管上皮、胃肠腺和肾曲小管。该抗体还可用于检测肺癌患者血清中的大分子抗原，命名为KA - 32。血清中的抗原水平通过使用纯化的KM - 32进行抑制试验来测定。与健康成年人血清相比，超过一半的肺癌患者和良性疾病患者血清中的抑制水平更高。血清中抗原的纯化通过凝胶过滤色谱法、免疫亲和色谱法和聚丙烯酰胺梯度凝胶电泳进行。纯化后的抗原具有糖蛋白性质，其分子量估计超过500,000。
