Faculty of Architecture, Civil and Transportation Engineering, Beijing University of Technology, Beijing 100124, PR China.
Faculty of Architecture, Civil and Transportation Engineering, Beijing University of Technology, Beijing 100124, PR China.
Bioresour Technol. 2022 May;351:127016. doi: 10.1016/j.biortech.2022.127016. Epub 2022 Mar 16.
Effects of antibiotics (azithromycin, AZM, 1-40 mg/L) and quorum sensing inhibitor (QSI, 2(5H)-furanone, 1-40 mg/L) combined pollution with environmental concentration of copper on bacterial/archaeal community and antibiotic resistance genes (ARGs) in activated sludge system were explored. QSI inhibited nitrification more obviously than AZM. AZM and QSI were synergistic inhibitions on bacterial diversity, and AZM inhibited bacterial compositions more than QSI. While, QSI had more impacts on archaeal diversity/compositions. Less interactions among bacteria and archaea communities with Aquimonas as keystone genus. Functional differences in bacteria/archaea communities were little, and AZM had more effects on metabolism. AZM mainly affected nitrifying bacteria (Candidatus Nitrospira nitrificans and Nitrosomonas). Specific denitrifying bacteria were enriched by AZM (Brevundimonas, 1.76-31.69%) and QSI (Comamonas, 0.61-9.61%), respectively. AZM enriched ARGs more easily than QSI and they were antagonistic to proliferation of ARGs. Bacteria were main hosts of ARGs (macrolide-lincosamide-streptogramin B, other/efflux, etc.) and archaea (Methanosphaerula, Methanolobus) carried multiple ARGs.
研究了抗生素（阿奇霉素，AZM，1-40mg/L）和群体感应抑制剂（QSI，2（5H）-呋喃酮，1-40mg/L）联合污染与环境浓度铜对活性污泥系统中细菌/古菌群落和抗生素抗性基因（ARGs）的影响。QSI 对硝化的抑制作用比 AZM 更明显。AZM 和 QSI 对细菌多样性具有协同抑制作用，AZM 对细菌组成的抑制作用大于 QSI。然而，QSI 对古菌多样性/组成的影响更大。Aquimonas 作为关键属，细菌和古菌群落之间的相互作用较少。细菌/古菌群落的功能差异较小，而 AZM 对代谢的影响更大。AZM 主要影响硝化细菌（Candidatus Nitrospira nitrificans 和 Nitrosomonas）。特定的反硝化细菌分别被 AZM（Brevundimonas，1.76-31.69%）和 QSI（Comamonas，0.61-9.61%）富集。AZM 比 QSI 更容易富集 ARGs，且它们对抗 ARGs 的增殖具有拮抗作用。细菌是 ARGs 的主要宿主（大环内酯-林可酰胺-链阳性菌素 B、其他/外排等），而古菌（Methanosphaerula、Methanolobus）携带多种 ARGs。
