Faculty of Architecture, Civil and Transportation Engineering, Beijing University of Technology, Beijing 100124, PR China.
Faculty of Architecture, Civil and Transportation Engineering, Beijing University of Technology, Beijing 100124, PR China.
Bioresour Technol. 2022 Feb;345:126568. doi: 10.1016/j.biortech.2021.126568. Epub 2021 Dec 16.
The responses of microbial communities and antibiotic resistance genes (ARGs) to azithromycin and copper combined pollution under gradient increasing (from 0.5 to 10 mg/L) and decreasing exposure (from 10 to 0.5 mg/L) modes were investigated. Nitrification was inhibited more obviously under gradient increasing exposure mode. Responses of archaeal community and function structure were more obvious than bacteria under both exposure modes. The dominant bacterial and archaeal compositions (Hyphomicrobium, Euryarchaeota, etc.) were affected by two exposure modes, except some rare archaea (Methanoregula and Methanosarcina). There were more positive correlations between bacteria and archaea, and Nitrospira was keystone genus. Ammonia-oxidizing archaea (0.37-3.06%) and complete ammonia oxidizers (Nitrospira_ENR4) were enriched, and Nitrososphaera_viennensis was closely related to denitrifying genes (napA/B, nosZ, etc.). 50 ARG subtypes were detected and specific ARG subtypes (aac, ImrA, etc.) proliferated in two exposure modes. Bacteria and archaea were common hosts for 24 ARGs and contributed to their shifts.
研究了在梯度增加（从 0.5 到 10 mg/L）和减少暴露（从 10 到 0.5 mg/L）模式下，微生物群落和抗生素抗性基因（ARGs）对阿奇霉素和铜联合污染的响应。在梯度增加暴露模式下，硝化作用受到更明显的抑制。在两种暴露模式下，古菌群落和功能结构的响应比细菌更明显。除了一些稀有古菌（Methanoregula 和 Methanosarcina）外，两种暴露模式都影响了主要的细菌和古菌组成（Hyphomicrobium、广古菌门等）。细菌和古菌之间存在更多的正相关关系，硝化螺旋菌是关键属。氨氧化古菌（0.37-3.06%）和完全氨氧化菌（Nitrospira_ENR4）得到了富集，Nitrososphaera_viennensis 与反硝化基因（napA/B、nosZ 等）密切相关。检测到 50 种 ARG 亚型，两种暴露模式下特定的 ARG 亚型（aac、ImrA 等）增殖。细菌和古菌是 24 种 ARG 的共同宿主，对其转移起作用。
