Faculty of Architecture, Civil and Transportation Engineering, Beijing University of Technology, Beijing 100124, PR China.
Faculty of Architecture, Civil and Transportation Engineering, Beijing University of Technology, Beijing 100124, PR China.
Bioresour Technol. 2022 Apr;349:126882. doi: 10.1016/j.biortech.2022.126882. Epub 2022 Feb 22.
This study aimed to explore the impacts of ciprofloxacin (CIP, 0.05-40 mg/L) and copper (3 mg/L) combined pollution on nitrification, microbial community and antibiotic resistance genes (ARGs) in activated sludge system during stress- and post-effect periods. Higher CIP concentration inhibited nitrification and an average of 50% total nitrogen removal occurred under 40 mg/L of CIP pressure. The stress- and post-effects on bacterial diversity and structure were obviously distinct. Abundant genera were more sensitive to combined pollution than rare genera based on full-scale classification and conditionally rare or abundant taxa were keystone taxa in their interactions. Ammonia oxidation genes were inhibited under high CIP level, but some aerobic denitrifying bacteria (Thauera, Comamonas and Azoarcus) and key genes increased. 96 ARG subtypes were detected with complex positive relationships and their potential hosts (abundant-rare-functional genera) changed in two periods. This study highlights the different stress- and post-effects of combined pollution on activated sludge.
本研究旨在探讨在应激期和效应后期，环丙沙星（CIP，0.05-40mg/L）和铜（3mg/L）联合污染对活性污泥系统硝化作用、微生物群落和抗生素抗性基因（ARGs）的影响。较高浓度的 CIP 抑制了硝化作用，在 40mg/L 的 CIP 压力下，总氮去除率平均为 50%。细菌多样性和结构的应激和后效影响明显不同。基于全尺度分类，丰富属比稀有属对联合污染更敏感，条件稀有或丰富的分类群是相互作用中的关键分类群。在高 CIP 水平下，氨氧化基因受到抑制，但一些好氧反硝化细菌（Thauera、Comamonas 和 Azoarcus）和关键基因增加。检测到 96 种 ARG 亚型，它们之间存在复杂的正相关关系，其潜在宿主（丰富-稀有-功能属）在两个时期发生了变化。本研究强调了联合污染对活性污泥的不同应激和后效。
