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环状 RNA circ_0001006 通过 miR-214-3p/PAK6 轴加重心肌肥厚。

Circular RNA circ_0001006 aggravates cardiac hypertrophy via miR-214-3p/PAK6 axis.

机构信息

Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi 710061, PR China.

Key Laboratory of Molecular Cardiology, Xi'an Jiaotong University, Xi'an, Shanxi 710061, PR China.

出版信息

Aging (Albany NY). 2022 Mar 6;14(5):2210-2220. doi: 10.18632/aging.203461.

DOI:10.18632/aging.203461
PMID:35306484
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8954976/
Abstract

AIM

Circular RNAs (circRNAs) control gene expression in a series of physiological and pathological processes, but their role in heart disease is unknown. This research illustrates the role and potential mechanism of circRNA in cardiac hypertrophy.

METHODS AND RESULTS

In this report, we found that circular RNA hsa_circ_0001006 (circ_0001006) was upregulated in cardiac hypertrophy mice and cardiomyocytes treated with angiotensin II (Ang II). Next, we noticed that gain of function circ_0001006 could induce cardiomyocyte hypertrophy; oppositely, knockdown of circ_0001006 remitted Ang II-induced cardiomyocyte hypertrophy. Biotin-coupled miRNA and RNA-pull down assays showed that miR-214-3p could bind with circ_0001006 and gain the function of miR-214-3p abrogated the pro-hypertrophy effect of circ_0001006. Furthermore, Further, dual-luciferase reporter assay showed that miR-214-3p could interact with 3'UTRs of the PAK6 gene, and circRNA_0001006 could block the above interactions. Additionally, PAK6 expression is inhibited by miR-214-3p mimic in cardiomyocytes but enhanced by over-expression of circRNA_000203 .

CONCLUSIONS

Our data demonstrated that circRNA_0001006 exacerbates cardiac hypertrophy via suppressing miR-214-3p leading to enhanced PAK6 levels.

摘要

目的

环状 RNA（circRNAs）在一系列生理和病理过程中控制基因表达，但它们在心脏病中的作用尚不清楚。本研究阐述了 circRNA 在心肌肥厚中的作用和潜在机制。

方法和结果

在本报告中，我们发现环状 RNA hsa_circ_0001006（circ_0001006）在心肌肥厚小鼠和血管紧张素 II（Ang II）处理的心肌细胞中上调。接下来，我们注意到功能获得的 circ_0001006 可诱导心肌细胞肥大；相反，circ_0001006 的敲低可减轻 Ang II 诱导的心肌细胞肥大。生物素偶联 miRNA 和 RNA 下拉测定表明，miR-214-3p 可以与 circ_0001006 结合，并获得 miR-214-3p 的功能，从而消除了 circ_0001006 的促肥大作用。此外，双荧光素酶报告基因检测显示，miR-214-3p 可以与 PAK6 基因的 3'UTR 相互作用，而 circRNA_0001006 可以阻断上述相互作用。此外，miR-214-3p 模拟物在心肌细胞中抑制 PAK6 的表达，但增强 circRNA_000203 的表达则增强 PAK6 的表达。

结论

我们的数据表明，circRNA_0001006 通过抑制 miR-214-3p 导致 PAK6 水平升高，从而加重心肌肥厚。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/b1cf084ff809/aging-14-203461-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/8cb7b69e68b1/aging-14-203461-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/534e1c4f1a54/aging-14-203461-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/85a56cb190f4/aging-14-203461-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/ede2d51256b7/aging-14-203461-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/66cb18877df5/aging-14-203461-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/3b9098d85f9b/aging-14-203461-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/b1cf084ff809/aging-14-203461-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/8cb7b69e68b1/aging-14-203461-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/534e1c4f1a54/aging-14-203461-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/85a56cb190f4/aging-14-203461-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/ede2d51256b7/aging-14-203461-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/66cb18877df5/aging-14-203461-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/3b9098d85f9b/aging-14-203461-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ff/8954976/b1cf084ff809/aging-14-203461-g007.jpg

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本文引用的文献

1
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2
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J Thromb Thrombolysis. 2020 Apr;49(3):386-394. doi: 10.1007/s11239-019-01998-4.
3
Circ-HIPK3 Strengthens the Effects of Adrenaline in Heart Failure by MiR-17-3p - ADCY6 Axis.环状 RNA-HIPK3 通过 miR-17-3p-ADCY6 轴增强肾上腺素在心力衰竭中的作用。
Curr Top Med Chem. 2024;24(17):1451-1463. doi: 10.2174/0115680266300535240514104107.
4
Global Profile of tRNA-Derived Small RNAs in Pathological Cardiac Hypertrophy Plasma and Identification of tRF-21-NB8PLML3E as a New Hypertrophy Marker.病理性心肌肥厚血浆中tRNA衍生小RNA的全球概况及tRF-21-NB8PLML3E作为新的肥厚标志物的鉴定
Diagnostics (Basel). 2023 Jun 14;13(12):2065. doi: 10.3390/diagnostics13122065.
5
circRNA_0001006 predicts prognosis and regulates cellular processes of triple-negative breast cancer via miR-424-5p.环状RNA_0001006通过miR-424-5p预测三阴性乳腺癌的预后并调节其细胞进程。
Cell Div. 2023 May 16;18(1):7. doi: 10.1186/s13008-023-00089-4.
6
Noncoding RNAs as Key Regulators for Cardiac Development and Cardiovascular Diseases.非编码RNA作为心脏发育和心血管疾病的关键调节因子
J Cardiovasc Dev Dis. 2023 Apr 12;10(4):166. doi: 10.3390/jcdd10040166.
7
Insights into circular RNAs: Biogenesis, function and their regulatory roles in cardiovascular disease.环状 RNA 的研究进展：生物发生、功能及其在心血管疾病中的调控作用。
J Cell Mol Med. 2023 May;27(10):1299-1314. doi: 10.1111/jcmm.17734. Epub 2023 Apr 1.
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4
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6
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8
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9
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