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对Analytab肠杆菌(API 20E)系统进行标准化,以提高细菌生物型鉴定试验的准确性和可重复性。

Standardization of the Analytab Enteric (API 20E) system to increase accuracy and reproducibility of the test for biotype characterization of bacteria.

作者信息

Murray P R

出版信息

J Clin Microbiol. 1978 Jul;8(1):46-9. doi: 10.1128/jcm.8.1.46-49.1978.

Abstract

Procedures employing the Analytab Enteric (API 20E) system were standardized to improve the accuracy and reproducibility of the individual biochemical tests so that the system could be used to biochemically characterize bacteria for epidemiological studies. The standardized method and the method recommended by the manufacturer (routine method) were tested in parallel with 130 clinical isolates. Tests with 100 randomly selected clinical isolates demonstrated that the standardized method was more accurate and reproducible than the routine method. In addition, the standardized method accurately identified 24 of 30 clinical isolates which could not be identified with the routine method.

摘要

采用Analytab肠道(API 20E)系统的程序进行了标准化,以提高各个生化试验的准确性和可重复性,从而使该系统可用于对细菌进行生化特性分析,以开展流行病学研究。将标准化方法与制造商推荐的方法(常规方法)同时用于130株临床分离株进行测试。对100株随机选择的临床分离株进行的测试表明,标准化方法比常规方法更准确、更具可重复性。此外,标准化方法准确鉴定出了30株临床分离株中的24株,而常规方法无法鉴定这些分离株。

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