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API 20E系统的误用及实验室间测试的可重复性

Misuse and interlaboratory test reproducibility of API 20E system.

作者信息

Holmes B, Dawson C A

出版信息

J Clin Pathol. 1985 Aug;38(8):937-41. doi: 10.1136/jcp.38.8.937.

DOI:10.1136/jcp.38.8.937
PMID:3897293
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC499401/
Abstract

One hundred strains were referred to us for identification because they apparently could not be identified satisfactorily with the API 20E system (appareils et procédés d'identification). The inability to identify 31 strains was due primarily to failure to follow the manufacturer's instructions. Twenty six further strains were found to have been correctly identified by the sender's own API 20E results, so that only the remaining 43 strains definitely fell into the category for which our identification service was intended. Eighteen of the 43 strains not identified by the sender were identified by us using the API 20E system, and several possible reasons are given to explain the differences in these results. The remaining 25 strains either could not be identified by us on the API 20E system or, in the case of 13, they could not be identified by our conventional system and therefore no comparison could be made. The average interlaboratory probability of errors for the API 20E tests was 6.1%.

摘要

有100株菌株被送到我们这里进行鉴定,因为它们显然无法通过API 20E系统(鉴定仪器和方法)得到令人满意的鉴定结果。31株菌株无法鉴定主要是因为未遵循制造商的说明。另外26株菌株经发送方自己的API 20E结果显示已被正确鉴定,所以只有剩下的43株菌株确实属于我们鉴定服务所针对的类别。发送方未鉴定出的43株菌株中有18株被我们用API 20E系统鉴定出来了,文中给出了几个可能的原因来解释这些结果的差异。剩下的25株菌株要么我们无法用API 20E系统鉴定出来,要么对于其中13株,我们的传统系统也无法鉴定,因此无法进行比较。API 20E测试的实验室间平均误差概率为6.1%。

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本文引用的文献

1
The systems approach to diagnostic microbiology.诊断微生物学的系统方法。
Crit Rev Microbiol. 1981;9(1):1-44. doi: 10.3109/10408418109104485.
2
A review of numerical methods in bacterial identification.细菌鉴定中的数值方法综述。
Antonie Van Leeuwenhoek. 1980;46(3):233-99. doi: 10.1007/BF00453024.
3
Identification of bacteria by computer: general aspects and perspectives.利用计算机鉴定细菌:总体情况与展望。
J Gen Microbiol. 1973 Aug;77(2):273-90. doi: 10.1099/00221287-77-2-273.
4
Carbon source utilization tests as an aid to the classification of non-fermenting gram-negative bacteria.碳源利用试验辅助非发酵革兰氏阴性菌的分类
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5
Reproducibility of the analytab (API 20E) system.安纳塔布(API 20E)系统的可重复性。
J Clin Microbiol. 1975 Oct;2(4):322-6. doi: 10.1128/jcm.2.4.322-326.1975.
6
Standardization of the Analytab Enteric (API 20E) system to increase accuracy and reproducibility of the test for biotype characterization of bacteria.对Analytab肠杆菌(API 20E)系统进行标准化,以提高细菌生物型鉴定试验的准确性和可重复性。
J Clin Microbiol. 1978 Jul;8(1):46-9. doi: 10.1128/jcm.8.1.46-49.1978.
7
Identification of Enterobacteriaceae by the API 20E system.使用API 20E系统鉴定肠杆菌科细菌。
J Clin Pathol. 1978 Jan;31(1):22-30. doi: 10.1136/jcp.31.1.22.
8
Test reproducibility of the API (20E), Enterotube, and Pathotec systems.测试API(20E)、肠杆菌鉴定管和Pathotec系统的可重复性。
J Clin Pathol. 1977 Apr;30(4):381-7. doi: 10.1136/jcp.30.4.381.