Chang Xuefei, Bi Yaluan, Chi Haipeng, Fang Qi, Lu Zhaozhi, Wang Fang, Ye Gongyin
Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China.
State Key Laboratory of Rice Biology & Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Diseases and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
Insects. 2022 Nov 5;13(11):1024. doi: 10.3390/insects13111024.
The insect odorant binding proteins (OBPs) and chemosensory proteins (CSPs) are involved in the perception and discrimination of insects to host odor cues. , one of the destructive pests of rice plants, not only directly damages hosts by sucking, but also indirectly transmits plant viruses in the field. Previous study found that two rice volatiles (()-β-caryophyllene and 2-heptanol) induced by rice dwarf virus (RDV) mediated the olfactory behavior of , which may promote virus dispersal. However, the OBPs and CSPs in are still unknown. In this study, to identify the OBP and CSP genes in , transcriptomic analyses were performed. In total, 46,623 unigenes were obtained. Twenty putative OBP and 13 CSP genes were discovered and identified. Phylogenetic analyses revealed that five putative OBPs belonged to the plus-C OBP family, and the other classic OBPs and CSPs were distributed among other orthologous groups. A total of 12 OBP and 10 CSP genes were detected, and nine OBP and three CSP genes were highly expressed in antennae compared with other tissues. This study, for the first time, provides a valuable resource to well understand the molecular mechanism of in the perception and discrimination of the two volatiles induced by RDV infection.
昆虫气味结合蛋白（OBPs）和化学感受蛋白（CSPs）参与昆虫对寄主气味线索的感知和辨别。褐飞虱是水稻的毁灭性害虫之一，不仅通过吸食直接损害寄主，还在田间间接传播植物病毒。先前的研究发现，水稻矮缩病毒（RDV）诱导的两种水稻挥发物（（-）-β-石竹烯和2-庚醇）介导了褐飞虱的嗅觉行为，这可能促进病毒传播。然而，褐飞虱中的OBPs和CSPs仍不清楚。在本研究中，为了鉴定褐飞虱中的OBP和CSP基因，进行了转录组分析。总共获得了46,623个单基因。发现并鉴定了20个假定的OBP基因和13个CSP基因。系统发育分析表明，5个假定的OBP属于加C OBP家族，其他经典的OBP和CSP分布在其他直系同源组中。总共检测到12个OBP基因和10个CSP基因，与其他组织相比，9个OBP基因和3个CSP基因在褐飞虱触角中高表达。本研究首次提供了宝贵的资源，有助于深入了解褐飞虱对RDV感染诱导的两种挥发物的感知和辨别分子机制。
