State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing, 100049, China.
Jiangxi Provincial Key Laboratory of Water Resources and Environment of Poyang Lake, Jiangxi Academy of Water Science and Engineering, Nanchang, Jiangxi, 330029, China.
Environ Pollut. 2022 Jun 15;303:119180. doi: 10.1016/j.envpol.2022.119180. Epub 2022 Mar 17.
Antibiotics usage in animal production is considered a primary driver of the occurrence, supply and spread of antibiotic resistance genes (ARGs) in the environment. Pig farms and fish ponds are important breeding systems in food animal production. In this study, we compared and analyzed broad ARGs profiles, mobile genetic elements (MGEs) and bacterial communities in a representative pig farm and neighboring fish ponds around Poyang Lake, the largest freshwater lake in China. The factors influencing the distribution of ARGs were also explored. The results showed widespread detection of ARGs (from 57 to 110) among 283 targeted ARGs in the collected water samples. The differences in the number and relative abundance of ARGs observed from the pig farm and neighboring fish ponds revealed that ARG contamination was more serious on the pig farm than in the fish ponds and that the water treatment plant on the pig farm was not very effective. Based on the variance partition analysis (VPA), MGEs, bacterial communities and water quality indicators (WIs) codrive the relative abundance of ARGs. Based on network analysis, we found that total phosphorus and Tp614 were the most important WIs and MGEs affecting ARG abundance, respectively. Our findings provide fundamental data on farms in lakeside districts and provide insights into establishing standards for the discharge of aquaculture wastewater.
在动物生产中使用抗生素被认为是环境中抗生素耐药基因（ARGs）发生、供应和传播的主要驱动因素。养猪场和鱼塘是食品动物生产中的重要养殖系统。在本研究中，我们比较和分析了中国最大的淡水湖鄱阳湖周边一个具有代表性的养猪场和相邻鱼塘的广谱 ARGs 谱、移动遗传元件（MGEs）和细菌群落。还探讨了影响 ARGs 分布的因素。结果表明，在所采集的水样中，283 个靶向 ARGs 中广泛检测到 ARGs（从 57 个到 110 个）。从养猪场和相邻鱼塘中观察到的 ARGs 的数量和相对丰度的差异表明，养猪场的 ARG 污染比鱼塘严重，而且养猪场的水处理厂效果不是很好。基于方差分解分析（VPA），MGEs、细菌群落和水质指标（WIs）共同驱动 ARGs 的相对丰度。基于网络分析，我们发现总磷和 Tp614 是影响 ARG 丰度的最重要的 WI 和 MGEs。我们的研究结果为湖滨地区的农场提供了基础数据，并为制定水产养殖废水排放标准提供了思路。
