National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, People's Republic of China.
School of Agricultural Science and Engineering, Liaocheng University, No.1 Hunan Road, Liaocheng, 252000, Shandong, China.
Appl Microbiol Biotechnol. 2024 Dec;108(1):136. doi: 10.1007/s00253-023-12842-4. Epub 2024 Jan 15.
Livestock farms are major reservoirs of antibiotic resistance genes (ARGs) that are discharged into the environment. However, the abundance, diversity, and transmission of ARGs in duck farms and its impact on surrounding environments remain to be further explored. Therefore, the characteristics of ARGs and their bacterial hosts from duck farms and surrounding environment were investigated by using metagenomic sequencing. Eighteen ARG types which consist of 823 subtypes were identified and the majority conferred resistance to multidrug, tetracyclines, aminoglycosides, chloramphenicols, MLS, and sulfonamides. The floR gene was the most abundant subtype, followed by sul1, tetM, sul2, and tetL. ARG abundance in fecal sample was significantly higher than soil and water sample. Our results also lead to a hypothesis that Shandong province have been the most contaminated by ARGs from duck farm compared with other four provinces. PcoA results showed that the composition of ARG subtypes in water and soil samples was similar, but there were significant differences between water and feces samples. However, the composition of ARG subtypes were similar between samples from five provinces. Bacterial hosts of ARG subtypes were taxonomically assigned to eight phyla that were dominated by the Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria. In addition, some human bacterial pathogens could be enriched in duck feces, including Enterococcus faecium, Acinetobacter baumannii, and Staphylococcus aureus, and even serve as the carrier of ARGs. The combined results indicate that a comprehensive overview of the diversity and abundance of ARGs, and strong association between ARGs and bacterial community shift proposed, and benefit effective measures to improve safety of antibiotics use in livestock and poultry farming. KEY POINTS: • ARG distribution was widespread in the duck farms and surroundings environment • ARG abundance on the duck farms was significantly higher than in soil and water • Human bacterial pathogens may serve as the vectors for ARGs.
养殖场是抗生素耐药基因（ARGs）的主要储存库，这些基因会被排放到环境中。然而，鸭养殖场中 ARGs 的丰度、多样性和传播及其对周围环境的影响仍有待进一步探索。因此，本研究采用宏基因组测序的方法，研究了鸭养殖场及其周围环境中 ARG 的特征及其细菌宿主。共鉴定出 18 种 ARG 类型，包含 823 个亚型，这些亚型主要对抗生素具有耐药性，包括多药、四环素类、氨基糖苷类、氯霉素类、MLS 和磺胺类。其中，floR 基因为最丰富的亚型，其次是 sul1、tetM、sul2 和 tetL。粪便样本中的 ARG 丰度显著高于土壤和水样。我们的结果还表明，与其他四个省份相比，山东省的鸭养殖场受到 ARG 的污染最为严重。PcoA 结果表明，水样和土样中 ARG 亚型的组成相似，但水样和粪便样之间存在显著差异。然而，五个省份的样本中 ARG 亚型的组成相似。ARG 亚型的细菌宿主被分类为八个门，其中以变形菌门、厚壁菌门、拟杆菌门和放线菌门为主。此外，一些人类致病菌可能在鸭粪便中富集，包括屎肠球菌、鲍曼不动杆菌和金黄色葡萄球菌，甚至可能成为 ARGs 的载体。综合结果表明，该研究提出了鸭养殖场及其周围环境中 ARG 的多样性和丰度的综合概述，以及 ARGs 与细菌群落变化之间的强烈关联，这有利于采取有效措施改善畜禽养殖中抗生素的使用安全。关键点：
Appl Microbiol Biotechnol. 2024-12
Sci Total Environ. 2022-5-1
Environ Pollut. 2022-1-1
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