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铁皮石斛多糖改善链脲佐菌素诱导的糖尿病大鼠的睾丸生精功能。

Dendrobium nobile Lindl polysaccharides improve testicular spermatogenic function in streptozotocin-induced diabetic rats.

作者信息

Lei Xiaocan, Huo Peng, Xie Yuan-Jie, Wang Yaohui, Liu Guanghai, Tu Haoyan, Shi Qingxiang, Mo Zhong-Cheng, Zhang Shun

机构信息

Department of Histology and Embryology, Clinical Anatomy & Reproductive Medicine Application Institute, University of South China, Hengyang, China.

School of Public and Health, Guilin Medical University, Guilin, China.

出版信息

Mol Reprod Dev. 2022 Apr;89(4):202-213. doi: 10.1002/mrd.23556. Epub 2022 Mar 20.

Abstract

Dendrobium nobile Lindl polysaccharides (DNLP) exhibited various biological functions. This study aimed to investigate the protective effects of DNLP on testicular spermatogenic function in streptozotocin (STZ)-induced diabetic rats in comparison with metformin. The blood glucose level was significantly increased and the homeostatic model assessment for insulin resistance (HOMA-IR) aggravated markedly in diabetic rats. The weight of testis and epididymis, and the sperm number and motility were decreased in the diabetic rats. The pathologic changes occurred in the spermatogenic tubules along with the decreased number of spermatogenic cells, downregulated proliferating cell nuclear antigen (PCNA) and Sirtuin 1 (SIRT1) expression and increased cell apoptosis in the testes. Compared with the model group, DNLP and metformin treatment significantly decreased the level of blood glucose, improved the HOMA-IR, and increased the weight of testis and epididymis, as well as the sperm number and sperm motility. Furthermore, the pathologic changes in the spermatogenic tubules improved significantly with increased number of spermatogenic cells, the upregulation of PCNA and SIRT1 and suppression of cell apoptosis in the testes. Collectively, our study for the first time examined the effects of DNLP on the male reproductive system of STZ-induced diabetic rats, and indicated that DNLP was protective against diabetes mellitus-induced testis injury via increasing the proliferation, inhibiting cell apoptosis and upregulating SIRT1 expression in testicular spermatogenic cells.

摘要

铁皮石斛多糖(DNLP)具有多种生物学功能。本研究旨在比较DNLP与二甲双胍对链脲佐菌素(STZ)诱导的糖尿病大鼠睾丸生精功能的保护作用。糖尿病大鼠的血糖水平显著升高,胰岛素抵抗的稳态模型评估(HOMA-IR)明显加重。糖尿病大鼠的睾丸和附睾重量、精子数量和活力均下降。生精小管出现病理变化,同时睾丸中生精细胞数量减少、增殖细胞核抗原(PCNA)和沉默信息调节因子1(SIRT1)表达下调以及细胞凋亡增加。与模型组相比,DNLP和二甲双胍治疗显著降低了血糖水平,改善了HOMA-IR,并增加了睾丸和附睾的重量以及精子数量和精子活力。此外,生精小管的病理变化显著改善,睾丸中生精细胞数量增加、PCNA和SIRT1上调以及细胞凋亡受到抑制。总体而言,我们的研究首次考察了DNLP对STZ诱导的糖尿病大鼠雄性生殖系统的影响,并表明DNLP通过增加睾丸生精细胞的增殖、抑制细胞凋亡和上调SIRT1表达对糖尿病诱导睾丸损伤具有保护作用。

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