人参皂苷 Rb1 通过 SDF-1/CXCR4 轴和 PI3K/Akt 通路调节骨髓间充质干细胞的迁移。
Ginsenoside Rb1 Modulates the Migration of Bone-Derived Mesenchymal Stem Cells through the SDF-1/CXCR4 Axis and PI3K/Akt Pathway.
机构信息
Department of Critical Care Medicine, Zhongshan Hospital Fudan University, Shanghai 200032, China.
Department of Facial Plastic and Reconstructive Surgery, Eye & ENT Hospital of Fudan University, Shanghai 200031, China.
出版信息
Dis Markers. 2022 Mar 10;2022:5196682. doi: 10.1155/2022/5196682. eCollection 2022.
METHODS
Wound-healing assay and Transwell assay were utilized to evaluate the effect of ginsenoside Rb1 on the migration of BMSCs. RT-PCR and Western blotting were performed to evaluate the expression of stromal-derived factor 1 (SDF-1), C-X-C chemokine receptor type 4 (CXCR4), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (PKB; AKT).
RESULTS
Ginsenoside Rb1 significantly enhanced the migration of BMSCs through the activation of SDF-1, CXCR4, p-PI3K/PI3K, and p-Akt/Akt relative expression. Furthermore, this stimulus was blocked by the pretreatment with AMD3100 and LY294002.
CONCLUSIONS
Ginsenoside Rb1 facilitated the migration of BMSCs through the activation of the SDF-1/CXCR4 axis and PI3K/Akt pathway.
方法
利用伤口愈合试验和 Transwell 试验来评估人参皂苷 Rb1 对骨髓间充质干细胞迁移的影响。采用 RT-PCR 和 Western blot 法来评估基质衍生因子 1（SDF-1）、C-X-C 趋化因子受体 4（CXCR4）、磷酸肌醇 3-激酶（PI3K）和蛋白激酶 B（PKB；AKT）的表达。
结果
人参皂苷 Rb1 通过激活 SDF-1、CXCR4、p-PI3K/PI3K 和 p-Akt/Akt 的相对表达，显著增强了骨髓间充质干细胞的迁移。此外，该刺激作用可被 AMD3100 和 LY294002 的预处理所阻断。
结论
人参皂苷 Rb1 通过激活 SDF-1/CXCR4 轴和 PI3K/Akt 通路促进骨髓间充质干细胞的迁移。
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