Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A&F University, Hangzhou, 311300, China.
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China.
Chem Biodivers. 2022 Sep;19(9):e202200506. doi: 10.1002/cbdv.202200506. Epub 2022 Aug 8.
The utilization of bamboo industry exhibits varied but still needs to be improved. Bamboo leaf flavonoid (BLF) is an important resource of bamboo which has become a research focus. However, the isolation and purification techniques of four flavonoid carbon glycosides (orientin, isoorientin, vitexin, and isovitexin) from BLF were still confronted with difficulties due to their complex and similar structures, which obstructed the development of bamboo utilization. In this article, a purification technology of four flavonoid carbon glycosides from BLF by Sephadex LH-20 was improved. The results were evaluated by HPLC and pharmacological activity. Specifically, the eluent, flow rate, and loading amount were investigated, respectively. According to the results, the eluent would dominate the isolation effect among three factors. High concentration of isoorientin and four flavonoid carbon glycosides would be obtained under the optimized condition (The eluent was 70 % methanol, the loading amount was 1.5 g, and the flow rate was 0.5 mL/min). Meanwhile, the link between flavonoid carbon glycosides content and their antioxidant activity in vitro was also revealed. Overall, the results suggested that BLF may serve as potential functional food additives and medicine.
竹产业的利用表现出多样性，但仍需改进。竹叶黄酮（BLF）是竹子的重要资源，已成为研究热点。然而，由于其复杂且相似的结构，BLF 中四种黄酮碳糖苷（荭草苷、异荭草苷、牡荆素和异牡荆素）的分离和纯化技术仍然存在困难，这阻碍了竹产业的发展。本文改进了用 Sephadex LH-20 从 BLF 中纯化四种黄酮碳糖苷的技术。通过 HPLC 和药理学活性对结果进行了评估。具体而言，分别考察了洗脱液、流速和上样量。结果表明，在三个因素中，洗脱液是分离效果的主要影响因素。在优化条件下（洗脱液为 70%甲醇，上样量为 1.5 g，流速为 0.5 mL/min），可以获得高浓度的异荭草苷和四种黄酮碳糖苷。同时，还揭示了黄酮碳糖苷含量与其体外抗氧化活性之间的关系。总的来说，这些结果表明 BLF 可能作为有潜力的功能性食品添加剂和药物。
